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Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG1 |
| Clone | Ks18.27 |
| Immunogen | Cytoskeletal preparation from tumor cell line MCF-7 |
| Purification | protein A affinity chromatography |
| Conjugate | unconjugated |
| Formulation | lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4) |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 1000 (IHC, 50 µl/slide) |
| Intended use | Research use only |
| Tested species reactivity | bovine, human (negative with mouse) |
Applications
| Tested applications | Tested dilutions |
|---|---|
| ELISA | assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:10 - 1:50 |
| Immunohistochemistry (IHC) - paraffin | 1:10 - 1:50 (protease treatment and/or microwave treatment recommended) |
| Western Blot (WB) | assay dependent |
Background
Details
Ks 18.27 represents an excellent marker to discriminate simple epithelia from those of different origin.
Polypeptide reacting: Mr 45,000 polypeptide (keratin K18; formerly also designated cytokeratin 18) of all simple type and complex epithelia.
Reactivity on cultured cell lines: MCF-7, HeLa. A-431, PLC, RT 112
Tumors specifically detected: All adeno-CA tested; undifferentiated CA; cervix CA; hepatocellular CA
Reference
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