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anti-Keratin K18 mouse monoclonal, Ks18.27, lyophilized, purified

The anti-Keratin K18 detects all simple epithelia and epithelium-derived neoplasms.
Cat. No.: 61034
Quantity:  50 µg
No. of experiments:  ≤ 1000 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone Ks18.27
Immunogen Cytoskeletal preparation from tumor cell line MCF-7
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 1000 (IHC, 50 µl/slide)
Intended use Research use only
Tested species reactivity bovine, human (negative with mouse)


Tested applications Tested dilutions
ELISA assay dependent
Immunohistochemistry (IHC) - frozen 1:10 - 1:50
Immunohistochemistry (IHC) - paraffin 1:10 - 1:50 (protease treatment and/or microwave treatment recommended)
Western Blot (WB) assay dependent



Ks 18.27 represents an excellent marker to discriminate simple epithelia from those of different origin.
Polypeptide reacting: Mr 45,000 polypeptide (keratin K18; formerly also designated cytokeratin 18) of all simple type and complex epithelia.
Reactivity on cultured cell lines: MCF-7, HeLa. A-431, PLC, RT 112
Tumors specifically detected: All adeno-CA tested; undifferentiated CA; cervix CA; hepatocellular CA


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