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Product description
| Host | guinea pig |
|---|---|
| Antibody Type | polyclonal |
| Immunogen | human keratin K19, purified from MCF-7 cells |
| Purification | stabilized antiserum |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide and 0.5% BSA |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 200 (IHC, 50 µl/slide); ≤ 60 (WB, 5 ml reaction volume) |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | bovine, human (negative with mouse and rat) |
| Stability | Guinea pig (GP68) |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | 1:100 |
| Immunohistochemistry (IHC) - frozen | 1:100 |
| Immunohistochemistry (IHC) - paraffin | 1:100 (microwave treatment recommended) |
| Western Blot (WB) | 1:3,000 |
Background
Details
The antiserum represents an excellent marker for all glandular type epithelia, useful for discrimination of glandular epithelial carcinoma from those of different origin.
No reaction with hepa-tocellular ca.! Polypeptide Reacting: Mr 40 000 polypeptide (keratin K19) of human glandular epithelia.
Reactive polypeptide: acidic human keratin K19 (Mr 40,000; formerly also designated cytokeratin 19)
Tumors detected: all adeno-carcinoma tested
Reference
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Species
Application
human
IHC-IF (paraffin)
