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Product description
Host | guinea pig |
---|---|
Antibody Type | polyclonal |
Immunogen | human keratin K19, purified from MCF-7 cells |
Purification | stabilized antiserum |
Conjugate | unconjugated |
Formulation | contains 0.09% sodium azide and 0.5% BSA |
Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
No. of experiments | ≤ 200 (IHC, 50 µl/slide); ≤ 60 (WB, 5 ml reaction volume) |
Note | centrifuge prior to opening |
Intended use | Research use only |
Tested species reactivity | bovine, human (negative with mouse and rat) |
Stability | Guinea pig (GP68) |
Applications
Tested applications | Tested dilutions |
---|---|
Immunocytochemistry (ICC)/ Immunofluorescence (IF) | 1:100 |
Immunohistochemistry (IHC) - frozen | 1:100 |
Immunohistochemistry (IHC) - paraffin | 1:100 (microwave treatment recommended) |
Western Blot (WB) | 1:3,000 |
Background
Details
The antiserum represents an excellent marker for all glandular type epithelia, useful for discrimination of glandular epithelial carcinoma from those of different origin.
No reaction with hepa-tocellular ca.! Polypeptide Reacting: Mr 40 000 polypeptide (keratin K19) of human glandular epithelia.
Reactive polypeptide: acidic human keratin K19 (Mr 40,000; formerly also designated cytokeratin 19)
Tumors detected: all adeno-carcinoma tested
Reference
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Species
Application
human
IHC-IF (paraffin)