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IHC of human liver (courtesy of J.Heß, University Hospital Heidelberg) Zoom

IHC of human liver (courtesy of J.Heß, University Hospital Heidelberg)

anti-Keratin K19 mouse monoclonal, Ks19.1 (A53-B/A2), prediluted, purified

Ks19.1 represents an excellent marker to discriminate glandular epithelial carcinoma from those of different origin. No reaction with hepatocellular carcinoma. For best performance, Microwave treatment is recommended.
highly_published external_validation
Cat. No.: 65010
Quantity:  5 mL
No. of experiments:  ≤ 100 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

$191.00
Excl. VAT, excl. Shipping Cost

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Specifications

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2a
Clone Ks19.1 (A53-B/A2)
Immunogen Keratin K19 of Mr 40 000; from cultured human MCF-7 cells
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation PBS pH 7.4 with 0.5% BSA and 0.09% sodium azide
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 100 (IHC, 50 µl/slide)
Intended use Research use only
Tested species reactivity human

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

Ks 19.1 represents an excellent marker to discriminate glandular epithelial carcinoma from those of different origin. No reaction with hepatocellular carcinoma.
Polypeptide reacting: Mr 40 000 polypeptide (keratin K19; formerly also designated cytokeratin 19) of human glandular epithelia.
The epitope has been localized on aa. 311-335 (QSQLSMKAALEDTLAETEARFGAQL) of the a-helical fragment.
Tumors specifically detected: All tested adenocarcinoma; cholangio ca. of liver; renal cell carcinoma; transitional cell ca. of the bladder; ovary ca.; squamous cell ca. of cervix, bronchus and lung (intermediate type); mesothelioma; carcinoid tumor of bronchus; breast ca.; thymoma.
Reactivity on cultured cell lines: MCF-7

Reference

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