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IHC of human colon (courtesy of J.Heß, University Hospital Heidelberg) Zoom

IHC of human colon (courtesy of J.Heß, University Hospital Heidelberg)

anti-Keratin K19 mouse monoclonal, Ks19.2 (Z105.6), lyophilized, purified

Ks19.2 represents an excellent marker to discriminate glandular epithelial carcinoma from those of different origin. No reaction with hepatocellular carcinoma.
application_confirmed highly_published external_validation
Cat. No.: 61029
Quantity:  50 µg

Delivery Time: usually 1-7 working days

$276.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

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61029S

anti-Keratin K19 mouse monoclonal, Ks19.2 (Z105.6), purified, sample (ready-to-use)

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anti-Keratin K19, Ks19.2 (Z105.6) + positive western blot control

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anti-Keratin K19 mouse monoclonal, Ks19.2 (Z105.6), liquid, purified

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anti-Keratin K19 mouse monoclonal, Ks19.2 (Z105.6), liquid, purified, small

0.2 mL (50 µg/mL)
$170.00
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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2b
Clone Ks19.2 (Z105.6) (also published as BM 19.21, MAK 19.21)
Immunogen Keratin K19 of Mr 40 000; from cultured human MCF-7 cells
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5 % BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
UniprotID P08727
Intended use Research use only
Tested species reactivity bovine, human, rabbit, rat (negative with mouse, woodchuck, chicken, xenopus)

Applications

Tested applications Tested dilutions
ELISA assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:10 - 1:50
Immunohistochemistry (IHC) - paraffin 1:10 - 1:50 (microwave treatment recommended)
Western Blot (WB) 1:50 - 1:500

Background

Details

Ks 19.2 represents an excellent marker to discriminate glandular epithelial carcinoma from those of different origin. No reaction with hepatocellular carcinoma! Polypeptide Reacting: Mr 40 000 polypeptide (keratin K19; formerly also designated cytokeratin 19) of human glandular epithelia. The epitope has been localized on aa. 352-368 (VRADSERQNQEYQRLMD) of the α-helical fragment.

Tumors specifically detected: All tested adenocarcinoma; cholangio carcinoma of liver; renal cell carcinoma; transitional cell carcinoma of the bladder; ovary carcinoma; squamous cell carcinoma of cervix, bronchus and lung (intermediate type); mesothelioma; carcinoid tumor of bronchus; breast carcinoma; thymoma

Reactivity on cultured cell lines: MCF-7

Reference

Reference

PublicationSpeciesApplication
Sawitza, I., Kordes, C., Götze, S., Herebian, D. & Häussinger, D. Bile acids induce hepatic differentiation of mesenchymal stem cells. Sci. Rep. 5, (2015). rat ICC-IF
Langbein, L. et al. Characterization of a Novel Human Type II Epithelial Keratin K1b, Specifically Expressed in Eccrine Sweat Glands. J. Invest. Dermatol. 125, 428–444 (2005). human IHC (frozen)
Höchtlen-Vollmar, W. et al. Occult epithelial tumor cells detected in bone marrow by an enzyme immunoassay specific for cytokeratin 19. Int. J. cancer 70, 396–400 (1997). human ELISA
Dittadi, R. et al. Standardization of assay for cytokeratin-related tumor marker CYFRA21.1 in urine samples. Clin. Chem. 42, 1634–8 (1996). human ELISA
Petersen, G., Song, D., Hügle-Dörr, B., Oldenburg, I. & Bautz, E. K. Mapping of linear epitopes recognized by monoclonal antibodies with gene-fragment phage display libraries. Mol. Gen. Genet. MGG 249, 425–31 (1995). epitope mapping
Van Der Gaast ’, A. et al. Evaluation of a new tumour marker in patients with non-small-cell lung cancer: Cyfra 21.1. Br. J. Cancer 69, 525–528 (1994). human ELISA
Sugama, Y. et al. Clinical usefulness of CYFRA assay in diagnosing lung cancer: measurement of serum cytokeratin fragment. Jpn. J. Cancer Res. 85, 1178–84 (1994). human ELISA
Grenier, J. et al. Cyfra 21-1, a new marker of lung cancer. Nucl. Med. Biol. 21, 471–476 (1994). human ELISA
Möbus, V. J. et al. Establishment of new ovarian and colon carcinoma cell lines: differentiation is only possible by cytokeratin analysis. Br. J. Cancer 69, 422–428 (1994). human ICC-IF
Moll, R. et al. The human gene encoding cytokeratin 20 and its expression during fetal development and in gastrointestinal carcinomas. Differentiation. 53, 75–93 (1993). human IHC (frozen)
Pujol, J. L. et al. Serum fragment of cytokeratin subunit 19 measured by CYFRA 21-1 immunoradiometric assay as a marker of lung cancer. Cancer Res. 53, 61–6 (1993). human ELISA
Stieber, P. et al. CYFRA 21-1. A new marker in lung cancer. Cancer 72, 707–13 (1993). human ELISA
Moll, I. & Moll, R. Comparative cytokeratin analysis of sweat gland ducts and eccrine poromas. Arch. Dermatol. Res. 283, 300–9 (1991). human IHC (frozen)
Bader, B. L. & Franke, W. W. Cell type-specific and efficient synthesis of human cytokeratin 19 in transgenic mice. Differentiation. 45, 109–18 (1990). human IHC (frozen)
Bosch, F. X. et al. Extensive changes in cytokeratin expression patterns in pathologically affected human gingiva. Virchows Arch. B. Cell Pathol. Incl. Mol. Pathol. 58, 59–77 (1989). human IHC (frozen)
Moll, I., Heid, H. & Moll, R. Cytokeratin analysis of pilomatrixoma: changes in cytokeratin-type expression during differentiation. J. Invest. Dermatol. 91, 251–7 (1988). human IHC (frozen)
Heid, H. W., Moll, I. & Franke, W. W. Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues. II. Differentiation. 37, 215–30 (1988). human IHC (frozen)
Heid, H. W., Moll, I. & Franke, W. W. Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues. I. Human and bovine hair follicles. Differentiation. 37, 137–57 (1988). human IHC (frozen)
Dockhorn-Dworniczak, B. et al. Patterns of expression of cytoskeletal proteins in human thyroid gland and thyroid carcinomas. Differentiation. 35, 53–71 (1987). human IHC (frozen)

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