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IHC of human colon (courtesy of J.Heß, University Hospital Heidelberg) Zoom

IHC of human colon (courtesy of J.Heß, University Hospital Heidelberg)

anti-Keratin K19 mouse monoclonal, Ks19.2 (Z105.6), lyophilized, purified

Ks19.2 represents an excellent marker to discriminate glandular epithelial carcinoma from those of different origin. No reaction with hepatocellular carcinoma.
application_confirmed highly_published external_validation
Cat. No.: 61029
Quantity:  50 µg
No. of experiments:  ≤ 1000 (IHC, 50 µl/slide); ≤ 100 (WB, 5 ml reaction volume)

Delivery Time: usually 1-7 working days

$276.00
Excl. VAT, excl. Shipping Cost

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Specifications

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61029S

anti-Keratin K19 mouse monoclonal, Ks19.2 (Z105.6), purified, sample (ready-to-use)

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anti-Keratin K19, Ks19.2 (Z105.6) + positive western blot control

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anti-Keratin K19 mouse monoclonal, Ks19.2 (Z105.6), liquid, purified

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anti-Keratin K19 mouse monoclonal, Ks19.2 (Z105.6), liquid, purified, small

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2b
Clone Ks19.2 (Z105.6) (also published as BM 19.21, MAK 19.21)
Immunogen Keratin K19 of Mr 40 000; from cultured human MCF-7 cells
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5 % BSA in PBS buffer, pH 7.4)
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
UniprotID P08727
No. of experiments ≤ 1000 (IHC, 50 µl/slide); ≤ 100 (WB, 5 ml reaction volume)
Intended use Research use only
Tested species reactivity bovine, human, rabbit, rat (negative with mouse, woodchuck, chicken, xenopus)

Applications

Tested applications Tested dilutions
ELISA assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:10 - 1:50
Immunohistochemistry (IHC) - paraffin 1:10 - 1:50 (microwave treatment recommended)
Western Blot (WB) 1:50 - 1:500

Background

Details

Ks 19.2 represents an excellent marker to discriminate glandular epithelial carcinoma from those of different origin. No reaction with hepatocellular carcinoma! Polypeptide Reacting: Mr 40 000 polypeptide (keratin K19; formerly also designated cytokeratin 19) of human glandular epithelia. The epitope has been localized on aa. 352-368 (VRADSERQNQEYQRLMD) of the α-helical fragment.

Tumors specifically detected: All tested adenocarcinoma; cholangio carcinoma of liver; renal cell carcinoma; transitional cell carcinoma of the bladder; ovary carcinoma; squamous cell carcinoma of cervix, bronchus and lung (intermediate type); mesothelioma; carcinoid tumor of bronchus; breast carcinoma; thymoma

Reactivity on cultured cell lines: MCF-7

Reference

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