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anti-Keratin K19 mouse monoclonal, Ks19.2 (Z105.6), supernatant

Ks19.2 represents an excellent marker to discriminate glandular epithelial carcinoma from those of different origin. No reaction with hepatocellular carcinoma.
Cat. No.: 65129
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€139.00
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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2b
Clone Ks19.2 (Z105.6)
Immunogen Keratin K19 of Mr 40 000; from cultured human MCF-7 cells
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, fish, human, rabbit, rat ((not with mouse, woodchuck, chicken, xenopus))

Applications

Tested applications Tested dilutions
ELISA assay dependent
Immunohistochemistry (IHC) - frozen ready-to-use
Western Blot (WB) assay dependent

Background

Details

Ks 19.2 represents an excellent marker to discriminate glandular epithelial CA from those of different origin. No reaction with hepatocellular CA!
Tumors specifically detected: All tested adeno CA; cholangio CA of liver; renal cell CA; transitional cell CA of the bladder; ovary CA; squamous cell CA of cervix, bronchus and lung (intermediate type); mesothelioma; carcinoid tumor of bronchus; breast CA; thymoma.
Polypeptide reacting: Mr 40 000 polypeptide (keratin K19; formerly also designated cytokeratin 19) of human glandular epithelia. The epitope has been localized on aa. 352-368 (VRADSERQNQEYQRLMD) of the α-helical fragment.
Reactivity on cultured cell lines: MCF-7, RT 112, Detroit 562, RPMI 2650, HT-29, SSC-12

Reference

Reference

PublicationSpeciesApplication
Kordes, C., Sawitza, I., Götze, S., Herebian, D. & Häussinger, D. Hepatic stellate cells contribute to progenitor cells and liver regeneration. J. Clin. Invest. 124, 5503–5515 (2014). rat IHC (frozen), ICC-IF
Langbein, L. et al. Characterization of a Novel Human Type II Epithelial Keratin K1b, Specifically Expressed in Eccrine Sweat Glands. J. Invest. Dermatol. 125, 428–444 (2005). human IHC (frozen)
Höchtlen-Vollmar, W. et al. Occult epithelial tumor cells detected in bone marrow by an enzyme immunoassay specific for cytokeratin 19. Int. J. cancer 70, 396–400 (1997). human ELISA
Dittadi, R. et al. Standardization of assay for cytokeratin-related tumor marker CYFRA21.1 in urine samples. Clin. Chem. 42, 1634–8 (1996). human ELISA
Petersen, G., Song, D., Hügle-Dörr, B., Oldenburg, I. & Bautz, E. K. Mapping of linear epitopes recognized by monoclonal antibodies with gene-fragment phage display libraries. Mol. Gen. Genet. MGG 249, 425–31 (1995). epitope mapping
Van Der Gaast ’, A. et al. Evaluation of a new tumour marker in patients with non-small-cell lung cancer: Cyfra 21.1. Br. J. Cancer 69, 525–528 (1994). human ELISA
Sugama, Y. et al. Clinical usefulness of CYFRA assay in diagnosing lung cancer: measurement of serum cytokeratin fragment. Jpn. J. Cancer Res. 85, 1178–84 (1994). human ELISA
Grenier, J. et al. Cyfra 21-1, a new marker of lung cancer. Nucl. Med. Biol. 21, 471–476 (1994). human ELISA
Möbus, V. J. et al. Establishment of new ovarian and colon carcinoma cell lines: differentiation is only possible by cytokeratin analysis. Br. J. Cancer 69, 422–428 (1994). human ICC-IF
Moll, R. et al. The human gene encoding cytokeratin 20 and its expression during fetal development and in gastrointestinal carcinomas. Differentiation. 53, 75–93 (1993). human IHC (frozen)
Pujol, J. L. et al. Serum fragment of cytokeratin subunit 19 measured by CYFRA 21-1 immunoradiometric assay as a marker of lung cancer. Cancer Res. 53, 61–6 (1993). human ELISA
Stieber, P. et al. CYFRA 21-1. A new marker in lung cancer. Cancer 72, 707–13 (1993). human ELISA
Moll, I. & Moll, R. Comparative cytokeratin analysis of sweat gland ducts and eccrine poromas. Arch. Dermatol. Res. 283, 300–9 (1991). human IHC (frozen)
Bader, B. L. & Franke, W. W. Cell type-specific and efficient synthesis of human cytokeratin 19 in transgenic mice. Differentiation. 45, 109–18 (1990). human IHC (frozen)
Bosch, F. X. et al. Extensive changes in cytokeratin expression patterns in pathologically affected human gingiva. Virchows Arch. B. Cell Pathol. Incl. Mol. Pathol. 58, 59–77 (1989). human IHC (frozen)
Moll, I., Heid, H. & Moll, R. Cytokeratin analysis of pilomatrixoma: changes in cytokeratin-type expression during differentiation. J. Invest. Dermatol. 91, 251–7 (1988). human IHC (frozen)
Heid, H. W., Moll, I. & Franke, W. W. Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues. II. Differentiation. 37, 215–30 (1988). human IHC (frozen)
Heid, H. W., Moll, I. & Franke, W. W. Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues. I. Human and bovine hair follicles. Differentiation. 37, 137–57 (1988). human IHC (frozen)
Dockhorn-Dworniczak, B. et al. Patterns of expression of cytoskeletal proteins in human thyroid gland and thyroid carcinomas. Differentiation. 35, 53–71 (1987). human IHC (frozen)

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