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Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG1 |
| Clone | RCK108 |
| Immunogen | Human bladder cell line T24 |
| Purification | hybridoma cell culture supernatant |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 400 (IHC, 50 µl/slide) |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | human, mammals (negative with mouse, rat) |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:10 - 1:20 |
| Immunohistochemistry (IHC) - paraffin | 1:10 - 1:20 (microwave treatment recommended) |
| Western Blot (WB) | assay dependent |
Background
Details
Mab RCK 108 specifically reacts with keratin K19 (formerly also designated cytokeratin 19; 40 kD protein in immunoblotting), found in most glandular simple type epithelia and tumors derived therefrom (adenocarcinoma), most squamous cell carcinoma.
Positive control: Glandular epithelia.
Reference
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Species
Application
human
WB
IHC (paraffin)
IHC (paraffin)
