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Product description
| Host | guinea pig |
|---|---|
| Antibody Type | polyclonal |
| Immunogen | synthetic peptide (N-terminal aa 2-23) of human keratin K2 |
| Purification | stabilized antiserum |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide and 0.5% BSA |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 400 (IHC, 50 µl/slide); ≤ 200 (WB, 5 ml reaction volume) |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | bovine, human, mouse |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:200 |
| Western Blot (WB) | 1:5,000 - 1:10,000 |
Background
Details
The antiserum represents an excellent marker to study terminal epidermal differentiation. The mab is reactive with epidermal cells in uppermost suprabasal layers including scalp, foot and sole. It recognizes individual cells within epidermis of tongue and mamille (co-localization with keratin K1). It is also strongly reactive on hyperkeratosis of diverse origin.
The mab does notreact with palate keratin K76.
Reactive polypeptide: Basic human keratin K2 (MW 65,852; formerly also designated cytokeratin 2e).
Antigen recognized in species:
- bovine: calf hoof epidermis, snooze epithelium; negative with tongue epithelium;
- mouse: heterogenously on foot sole epidermis only negative with epidermis of other body sites.
Reference
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