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anti-Keratin K2 mouse monoclonal, Ks2.342.7.4, supernatant

The antibody is reactive with epidermal cells in uppermost suprabasal layers incl. scalp, foot and sole.

Cat. No.: 65191
Quantity:  1 mL

Delivery Time: usually 1-7 working days

€143.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone Ks2.342.7.4
Immunogen synthetic peptide (N-terminal amino acids nos. 2-23) of human keratin K2 (MW 65,852)
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, human, mouse, rat

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:200
Immunohistochemistry (IHC) - frozen 1:100 (pretreatment with Triton-X100 recommended)
Immunohistochemistry (IHC) - paraffin 1:100 (microwave treatment recommended)
Western Blot (WB) 1:60,000

Background

Details

Ks 2.342.7.4 represents an excellent marker to study terminal epidermal differentiation. The mab is reactive with epidermal cells in uppermost suprabasal layers including scalp, foot and sole. It recognizes individual cells within epidermis of tongue and mamille (co-localization with keratin K10). It is also reactive on hyperkeratosis of diverse viral and genetic origin.The mab does not react with palate keratin K76.

The actual clone designation of this antibody is Ks2.342.7.4. The 2 last digits refer to the passage number of the cultured cells of clone Ks2.342. Passage 7.1 is used up. 

Reactive polypeptide (specificity): Basic human keratin K2 (MW 65,852; formerly also designated cytokeratin 2e).

Antigen recognized in species:

  • bovine: calf hoof epidermis, snooze epithelium; negative with tongue epithelium;
  • Mouse/rat: heterogenously on foot sole epidermis only,negative with epidermis of other body sites.

   

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