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anti-Keratin K2 mouse monoclonal, Ks2.398.3.1, supernatant

The antibody is reactive with epidermal cells in uppermost suprabasal layers incl. scalp, foot and sole. No reaction with palate keratin 76.
Cat. No.: 65177
Quantity:  1 mL

Delivery Time: usually 1-7 working days

€143.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone Ks2.398.3.1
Immunogen synthetic peptide (N-terminal amino acids nos. 2-23) of human keratin K2 (MW 65,852)
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human ( negative with bovine, mouse, rat, Xenopus laevis)

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:150
Immunohistochemistry (IHC) - paraffin 1:150 (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

Ks2.398.3.1 represents an excellent marker to study terminal epidermal differentiation. The mab is reactive with epidermal cells in uppermost supra­basal layers including scalp, foot and sole. It recognizes individual cells within epidermis of tongue and mamille (co-localization with keratin K10). It is also reactive on hyperkeratosis of diverse viral and genetic origin.

The mab does not react with palate keratin K76.

Reactive polypeptide: Basic human keratin K2 (MW 65,852; formerly also designated cytokeratin 2e).

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