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anti-Keratin K20 mouse monoclonal, IT-Ks20.10, FITC Conjugate

Excellent marker for gastrointestinal epithelium; specific for adeno CA of the colon, transitional cell CA of the bladder and Merkel cell tumors of the skin.
Cat. No.: 61454
Quantity:  250 µL (0.2 mg/mL)

Delivery Time: usually 1-7 working days

€346.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone IT-Ks20.10
Immunogen electrophoretically purified keratin K 20 from human intestinal mucosa
Purification affinity chromatography
Conjugate FITC
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage store at 2 – 8 °C
Tested species reactivity human, pig, rat (negative with mouse)

Applications

Tested applications Tested dilutions
Flow Cytometry (FACS) assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:10 – 1:20
Immunohistochemistry (IHC) - paraffin 1:10 – 1:20 (microwave treatment recommended)

Background

Details

IT-Ks 20.10 represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative.
Polypeptide recognized: protein IT (keratin K20; Mr 46 000; formerly also designated cytokeratin 20).
Reactivity on cultured cell lines: HT-29, LoVo, DLD-1, SW 1116, CaCo-2, RT-4

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