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anti-Keratin K20 mouse monoclonal, IT-Ks20.3, supernatant

Marker for gastrointestinal epithelium; specific for adenocarcinoma of the colon, transitional cell carcinoma of the bladder and Merkel cell tumors of the skin.
Cat. No.: 65132
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€196.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone IT-Ks20.3
Immunogen Electrophoretically purified keratin K20 from human intestinal mucosa
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human, pig (negative with rat or dog)

Applications

Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen ready-to-use
Western Blot (WB) assay dependent

Background

Details

IT-Ks 20.3 represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative.

Polypeptide recognized: protein IT (keratin K20; Mr 46 000; formerly also designated cytokeratin 20).

Reactivity on cultured cell lines: HT-29, LoVo, DLD-1, SW 1116, CaCo-2, RT-4

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