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Ks20.8 staining on mouse stomach FFPE tissue (1:100, image courtesy of J.Heß, University Hospital Heidelberg) Zoom

Ks20.8 staining on mouse stomach FFPE tissue (1:100, image courtesy of J.Heß, University Hospital Heidelberg)

anti-Keratin K20 mouse monoclonal, IT-Ks20.8, lyophilized, purified

Excellent marker for gastrointestinal epithelium; specific for adenocarcinoma of the colon, transitional cell carcinoma of the bladder and Merkel cell tumors of the skin.
Cat. No.: 61026
Quantity:  50 µg

Delivery Time: usually 1-7 working days

€311.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2a
Clone IT-Ks20.8
Immunogen electrophoretically purified keratin K20 from human intestinal mucosa
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human, mouse

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:10 – 1:100 (treatment with 0.02 % Triton X-100 recommended)
Immunohistochemistry (IHC) - paraffin 1:10 – 1:100 (protease treatment and/or microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

IT-Ks 20.8 represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative.
Polypeptide recognized: protein IT (keratin K20; Mr 46 000; formerly also designated cytokeratin 20).

Tested cultured cell lines: HT-29, LoVo, DLD-1, SW 1116, CaCo-2, RT-4

Reference

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