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Alternative Products
| Cat. No. | Product Name | Format | Quantity | Price |
|---|---|---|---|---|
| 65026 |
anti-Keratin K20 mouse monoclonal, IT-Ks20.8 |
prediluted, purified, ready-to-use | 5 mL |
$272.00
Excl. VAT, excl. Shipping Cost
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| 19026 |
anti-Keratin K20 mouse monoclonal, IT-Ks20.8 |
prediluted, purified | 1 mL |
$310.00
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| 690026 |
anti-Keratin K20 mouse monoclonal, IT-Ks20.8 |
liquid, purified | 1 mL (50 µg/mL) |
$425.00
Excl. VAT, excl. Shipping Cost
|
| 690026S |
anti-Keratin K20 mouse monoclonal, IT-Ks20.8 |
liquid, purified, small | 0.2 mL (50 µg/mL) |
$170.00
Excl. VAT, excl. Shipping Cost
|
Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG2a |
| Clone | IT-Ks20.8 |
| Immunogen | electrophoretically purified keratin K20 from human intestinal mucosa |
| Purification | protein A affinity chromatography |
| Conjugate | unconjugated |
| Formulation | lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4) |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 2000 (IHC, 50 µl/slide); ≤ 1000 (WB, 5 ml reaction volume) |
| Intended use | Research use only |
| Tested species reactivity | human, mouse |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | 1:10 – 1:100 (treatment with 0.02 % Triton X-100 recommended) |
| Immunohistochemistry (IHC) - paraffin | 1:10 – 1:100 (protease treatment and/or microwave treatment recommended) |
| Western Blot (WB) | 1:1,000 - 1:5,000 |
Background
Details
IT-Ks 20.8 represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative.
Polypeptide recognized: protein IT (keratin K20; Mr 46 000; formerly also designated cytokeratin 20).
Tested cultured cell lines: RT-4
Reference
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human
IHC (paraffin)
human
IHC (paraffin)
human
IHC (paraffin)
