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Product description
| Host | guinea pig |
|---|---|
| Antibody Type | polyclonal |
| Immunogen | Synthetic peptide of human keratin K25 (formerly also designated keratin K25irs1); aa 13-26 (C-PRPTTGSLRLYGG), coupled to KLH |
| Purification | stabilized antiserum |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide and 0.5% BSA |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 400 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume) |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | human |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | 1:100 - 1:200 |
| Western Blot (WB) | 1:2,000 |
Background
Details
Guinea pig polyclonal anti-Keratin K25 (K25irs1) antibody. Specific for human irs-specific keratin K25. The antiserum stains specifically the Henle, Huxley and cuticle layer of the inner root sheath (irs) of the hair follicle.
Negative on all other epithelia tested.
Reference
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