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Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG1 |
| Clone | AE5 |
| Immunogen | human epidermal keratin |
| Purification | affinity chromatography |
| Conjugate | unconjugated |
| Formulation | PBS with 0.09% sodium azide |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 200 (IHC, 50 µl/slide); ≤ 20 (WB, 5 ml reaction volume) |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | bovine, human, rabbit |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | 1:50 |
| Western Blot (WB) | 1:500 |
Background
Details
AE 5 represents an excellent marker for corneal type differentiation. Positive for epithelial cells of cornea, snout and some oral mucosa. This antibody has been used for studying corneal epithelial stem cells.
Polypeptide reacting: Mr 64 000 polypeptide (keratin K3; formerly also designated cytokeratin 3) of human corneal epithelium and keratin K76 (formerly also designated cytokeratin K2p) of palate epithelium.
Tested cultured cell lines: Rabbit corneal epithelial cells
Reference
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