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anti-Keratin K3/K76 mouse monoclonal, AE5, purified

Detects keratin K3/K76 present in cornea and palate epithelium.
Cat. No.: 61807
Quantity:  200 µg (1 mg/mL)
No. of experiments:  ≤ 200 (IHC, 50 µl/slide); ≤ 20 (WB, 5 ml reaction volume)

Delivery Time: usually 1-7 working days

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone AE5
Immunogen human epidermal keratin
Purification affinity chromatography
Conjugate unconjugated
Formulation PBS with 0.09% sodium azide
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 200 (IHC, 50 µl/slide); ≤ 20 (WB, 5 ml reaction volume)
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity bovine, human, rabbit


Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:50
Western Blot (WB) 1:500



AE 5 represents an excellent marker for corneal type differentiation. Positive for epithelial cells of cornea, snout and some oral mucosa. This antibody has been used for studying corneal epithelial stem cells.
Polypeptide reacting: Mr 64 000 polypeptide (keratin K3; formerly also designated cytokeratin 3) of human corneal epithelium and keratin K76 (formerly also designated cytokeratin K2p) of palate epithelium.
Tested cultured cell lines: Rabbit corneal epithelial cells


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