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human oesophagus (courtesy of L. Langbein) Zoom

human oesophagus (courtesy of L. Langbein)

anti-Keratin K4 guinea pig polyclonal, serum

Excellent marker to discriminate non-cornified squamous epithelia (esophagus, vagina, gingival) from those of different origin.
Cat. No.: GP-K4
Quantity:  100 µL

Delivery Time: usually 1-7 working days

€249.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host guinea pig
Antibody Type polyclonal
Immunogen synthetic peptide (C-KIISTTTL NKRR) of human keratin K4 coupled to KLH
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human, mouse

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:200
Immunohistochemistry (IHC) - paraffin 1:100 (microwave treatment recommended)
Western Blot (WB) 1:3,000

Background

Details

Excellent marker to discriminate non-cornified squamous epithelia (esophagus, vagina, gingival) from those of different origin. Recognizes keratin K4 in non-cornifying squamous epithelia.
Keratin K4 is mostly present in combination with keratin K13. In contrast to keratin K13, the superficial cells of the cornea are reactive with keratin K4. The antibody also stains ciliated pseudo-stratified epithelium of bronchi and, focally, ductal epithelial cells.

Reactive polypeptide: Human type II (basic) keratin K4 (Mr 59 000), formerly also designated cytokeratin 4.

Positive control: Squamous epithelium of esophagus, gingiva, palate, vagina (staining all suprabasal layers).

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