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Product description
| Host | guinea pig |
|---|---|
| Antibody Type | polyclonal |
| Immunogen | synthetic peptide (C-KIISTTTL NKRR) of human keratin K4 coupled to KLH |
| Purification | stabilized antiserum |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide and 0.5% BSA |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 400 (IHC, 50 µl/slide); ≤ 60 (WB, 5 ml reaction volume) |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | human |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:200 |
| Immunohistochemistry (IHC) - paraffin | 1:50 - 1:200 (microwave treatment recommended) |
| Western Blot (WB) | 1:3,000 |
Background
Details
Excellent marker to discriminate non-cornified squamous epithelia (esophagus, vagina, gingival) from those of different origin. Recognizes keratin K4 in non-cornifying squamous epithelia.
Keratin K4 is mostly present in combination with keratin K13. In contrast to keratin K13, the superficial cells of the cornea are reactive with keratin K4. The antibody also stains ciliated pseudo-stratified epithelium of bronchi and, focally, ductal epithelial cells.
Reactive polypeptide: Human type II (basic) keratin K4 (Mr 59 000), formerly also designated cytokeratin 4.
Positive control: Squamous epithelium of esophagus, gingiva, palate, vagina (staining all suprabasal layers).
Reference
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