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anti-Keratin K4 mouse monoclonal, 6B10, supernatant

6B10 recognizes keratin K4 in non-cornifying squamous epithelia and is mostly present in combination with keratin K13. In contrast to keratin K13, superficial cells of the cornea are reactive with keratin K4.
Cat. No.: 10525
Quantity:  1 mL

Delivery Time: usually 1-7 working days

€228.00
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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone 6B10
Immunogen human esophagus
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.1% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human (most mammals)

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:5 - 1:10

Background

Details

6B10 recognizes keratin K4 in non-cornifying squamous epithelia. Keratin K4 is mostly present in combination with keratin K13. In contrast to keratin K13, the superficial cells of the cornea are reactive with keratin K4. 6B10 also stains ciliated pseudo-stratified epithelium of bronchi and, focally, ductal epithelial cells. Polypeptide reacting: 59 kD keratin K4 (formerly also designated cytokeratin 4).

Positive control: squamous cell carcinomas

Reference

Reference

PublicationSpeciesApplication
Langbein, L. et al. New facets of keratin K77: Interspecies variations of expression and different intracellular location in embryonic and adult skin of humans and mice. Cell Tissue Res. 354, 793–812 (2013). human IHC (frozen)
Corver, W. E. et al. Four-color multiparameter DNA flow cytometric method to study phenotypic intratumor heterogeneity in cervical cancer. Cytometry 39, 96–107 (2000). human FACS
Schaafsma, H. E. & Ramaekers, F. C. Cytokeratin subtyping in normal and neoplastic epithelium: basic principles and diagnostic applications. Pathol. Annu. 29 Pt 1, 21–62 (1994). human IHC (frozen)
Moll, R. et al. The human gene encoding cytokeratin 20 and its expression during fetal development and in gastrointestinal carcinomas. Differentiation. 53, 75–93 (1993). human IHC (frozen)
Smedts, F. et al. Keratin Expression in Cervical Cancer. Am. J. Pathol. 141, 497–511 (1992). human IHC (frozen)
Smedts, F. et al. Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia. Am. J. Pathol. 136, 657–68 (1990). human IHC (frozen)
Bosch, F. X. et al. Extensive changes in cytokeratin expression patterns in pathologically affected human gingiva. Virchows Arch. B Cell Pathol. Incl. Mol. Pathol. 58, 59–77 (1989). human IHC (frozen)
Leube, R. et al. Molecular Characterization and Expression of the Stratification-related Cytokeratins 4 and 15 Isolation of cDNA Clones Encoding. J. Cell Biol. 106, 1249–1261 (1988). human IHC (frozen)
Moll, R. et al. Cytokeratins in normal and malignant transitional epithelium: maintenance of expression of urothelial differentiation features in transitional cell carcinomas and bladder carcinoma cell culture lines. Am. J. Pathol. 132, 123–144 (1988). human IHC (frozen), ICC-IF
Van Muijen, G. N., Warnaar, S. O. & Ponec, M. Differentiation-related changes of cytokeratin expression in cultured keratinocytes and in fetal, newborn, and adult epidermis. Exp. Cell Res. 171, 331–45 (1987). human WB, IHC (frozen), ICC-IF

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