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anti-Keratin K5/K8 (Pan Epithelial) mouse monoclonal, C22, Biotin Conjugate

Decorates all epithelia and epithelium-derived neoplasms. C22 represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors.
Cat. No.: 61531
Quantity:  250 µL
No. of experiments:  ≤ 100 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

$377.00
Excl. VAT, excl. Shipping Cost

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Specifications

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone Ks 5+8.22/C22
Immunogen human keratin K8, purified from SDS PAGE gel
Purification protein A affinity chromatography
Conjugate biotin
Formulation contains 0.09% sodium azide
Storage store at 2-8°C
No. of experiments ≤ 100 (IHC, 50 µl/slide)
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity amphibia, bovine, human, hydra, mouse, pig

Applications

Tested applications Tested dilutions
ELISA assay dependent
Flow Cytometry (FACS) assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:10 – 1:20
Immunohistochemistry (IHC) - paraffin 1:10 – 1:20 (protease treatment and/or microwave treatment recommended)

Background

Details

C 22 represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. The antibody specifically reacts with keratins K5 and K8 present in nearly all epithelia. Polypeptide reacting: Mr 52 500, Mr 58 000 keratins (type II keratins K5 and K8; formerly also designated cytokeratins 5 and 8) of human epithelial cells.
Epitope has been mapped to aa 353-367 on alpha helical rod domain of Keratin K8 (Waseem et al. 2004).
Tested cultured cell lines: MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650, SSC-12, bovine BMGE+H, BMGE-H, MDBK

Waseem, A. et al. Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability. Biochemistry 43, 1283–95 (2004).

Reference

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