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anti-Keratin K5/K8 (Pan Epithelial) mouse monoclonal, C22, FITC Conjugate

Decorates all epithelia and epithelium-derived neoplasms. C22 represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors.
Cat. No.: 61431
Quantity:  250 µL

Delivery Time: usually 1-7 working days

€249.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone Ks 5+8.22/C22
Immunogen human keratin K8, purified from SDS PAGE gel
Purification protein A affinity chromatography
Conjugate FITC
Formulation contains 0.09% sodium azide
Storage store at 2 – 8 °C
UniprotID P05787
Tested species reactivity amphibia, bovine, human, hydra, mouse, pig

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:10
Immunohistochemistry (IHC) - paraffin 1:10 (protease treatment and microwave treatment recommended)

Background

Details

C 22 represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. The antibody specifically reacts with keratins K5 and K8 present in nearly all epithelia. Polypeptide reacting: Mr 52 500, Mr 58 000 keratins (type II keratins K5 and K8; formerly also designated cytokeratins 5 and 8) of human epithelial cells. Epitope has been mapped to aa 353-367 on alpha helical rod domain (Waseeman et al. 2004)

Tested cultured cell lines: MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650, SSC-12, bovine BMGE+H, BMGE-H, MDBK



Waseem, A. et al. Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability. Biochemistry 43, 1283–95 (2004).

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