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anti-Keratin K5/K8 (Pan Epithelial) mouse monoclonal, C22, prediluted, purified

Decorates all epithelia and epithelium-derived neoplasms. C22 represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors.
Cat. No.: 65031
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€139.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone C22
Immunogen human keratin K8, purified from SDS PAGE gel
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation PBS pH 7.4 with 0.5% BSA and 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity amphibia, bovine, human, hydra, mouse, pig

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (protease treatment and/or microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

C 22 represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. The antibody specifically reacts with keratins K5 and K8 present in nearly all epithelia. Polypeptide reacting:Mr 52 500, Mr 58 000 keratins (type II keratins K5 and K8; formerly also designated cytokeratins 5 and 8) of human epithelial cells.
Epitope has been mapped to aa 353-367 on alpha helical rod domain (see Waseem et al., 2004)
Reactivity on cultured cell lines: MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650, SSC-12, bovine BMGE+H, BMGE-H, MDBK

Reference

Reference

PublicationSpeciesApplication
Heid, H. et al. Lipid droplets, perilipins and cytokeratins--unravelled liaisons in epithelium-derived cells. PLoS One 8, (2013). human ICC-IF

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