human skin (courtesy of J.Heß, University Hospital Heidelberg)

anti-Keratin K6 mouse monoclonal, KA12, lyophilized, purified

Excellent marker for non-keratinized squamous epithelia and proliferating cells of epidermis (e.g. within psoriatic lesions).

Cat. No.: 61090
Quantity: 50 µg
No. of experiments: ≤ 1000 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

$251.00

Excl. VAT, excl. Shipping Cost

Qty:

Publication

Species

Application

Boink, M. et al. Comparison of advanced therapy medicinal product gingiva and skin substitutes and their in vitro wound healing potentials. J.Tissue.Eng.Regen.Med. 12, e1088-e1097 (2018).

human

IHC (paraffin)

Kist, R. et al. The Formation of Endoderm-Derived Taste Sensory Organs Requires a Pax9-Dependent Expansion of Embryonic Taste Bud Progenitor Cells. PLoS Genet. 10, (2014).

mouse

IHC (frozen)

Wallace, L., Roberts-Thompson, L. & Reichelt, J. Deletion of K1/K10 does not impair epidermal stratification but affects desmosomal structure and nuclear integrity. J. Cell Sci. 125, 1750â1758 (2012).

mouse

IHC (frozen)

Langbein, L., Yoshida, H., Praetzel-Wunder, S., Parry, D. A. & Schweizer, J. The Keratins of the Human Beard Hair Medulla: The Riddle in the Middle. J. Invest. Dermatol. 130, 55â73 (2010).

human

IHC (frozen)

Langbein, L., Eckhart, L., Rogers, M. A., Praetzel-Wunder, S. & Schweizer, J. Against the rules: Human keratin K80 - Two functional alternative splice variants, K80 and K80.1, with special cellular localization in a wide range of epithelia. J. Biol. Chem.

human

IHC (frozen)

Langbein, L. et al. Characterization of a Novel Human Type II Epithelial Keratin K1b, Specifically Expressed in Eccrine Sweat Glands. J. Invest. Dermatol. 125, 428â444 (2005).

human

IHC (frozen)

Herrmann, T. et al. Mice with targeted disruption of the fatty acid transport protein 4 (Fatp 4, Slc27a4) gene show features of lethal restrictive dermopathy. J. Cell Biol. 161, 1105â1115 (2003).

mouse

IHC (paraffin)

Langbein, L., Rogers, M. A., Praetzel, S., Winter, H. & Schweizer, J. K6irs1, K6irs2, K6irs3, and K6irs4 represent the inner-root-sheath-specific type II epithelial keratins of the human hair follicle. J. Invest. Dermatol. 120, 512â522 (2003).

human

Langbein, L. et al. A novel epithelial keratin, hK6irs1, is expressed differentially in all layers of the inner root sheath, including specialized huxley cells (FlÃ¼gelzellen) of the human hair follicle. J. Invest. Dermatol. 118, 789â799 (2002).

human

Demirkesen, C., Hoede, N. & Moll, R. Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins. J. Cutan. Pathol. 22, 518â35 (1995).

human

IHC (paraffin)

Smedts, F. et al. Keratin expression in cervical cancer. Am. J. Pathol. 141, 497â511 (1992).

human

IHC (frozen)

Wetzels, R. H. W. et al. Basal Cell-specific and Hyperproliferation-related Keratins in Human Breast Cancer. Am. J. Pathol. 138, 751â763 (1991).

human

IHC (frozen)

Alternative Products

Cat. No.	Product Name	Format	Quantity	Price
19090	anti-Keratin K6 mouse monoclonal, KA12	prediluted, purified	1 mL	$248.00 Excl. VAT, excl. Shipping Cost

Product description

Host	mouse
Antibody Type	monoclonal
Isotype	IgG1
Clone	Ks6.KA12
Immunogen	Keratin K6 of human callus cytoskeletal preparation
Purification	protein A affinity chromatography
Conjugate	unconjugated
Formulation	lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage	short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
UniprotID	P02538
No. of experiments	≤ 1000 (IHC, 50 µl/slide)
Intended use	Research use only
Tested species reactivity	human, mouse, rat

Applications

Tested applications	Tested dilutions
Immunohistochemistry (IHC) - frozen	1:5 - 1:50
Immunohistochemistry (IHC) - paraffin	1:5 - 1:50 (microwave treatment recommended)
Western Blot (WB)	assay dependent

Background

Details

Ks6.KA12 represents an excellent marker for non-keratinized squamous epithelia and proliferating cells of epidermis (e.g. within psoriatic lesions).

Polypeptide reacting: Mr 56 000 polypeptide (keratin K6; formerly also designated cytokeratin 6) of human squamous epithelia.

Tissues and Tumors specifically detected: The antibody is suitable for discrimination of keratinizing and non-keratinizing squamous cell carcinoma versus e.g. poorly differentiated adenocarcinoma.

Reference

Publication

Species

Application

Boink, M. et al. Comparison of advanced therapy medicinal product gingiva and skin substitutes and their in vitro wound healing potentials. J.Tissue.Eng.Regen.Med. 12, e1088-e1097 (2018).

human

IHC (paraffin)

Kist, R. et al. The Formation of Endoderm-Derived Taste Sensory Organs Requires a Pax9-Dependent Expansion of Embryonic Taste Bud Progenitor Cells. PLoS Genet. 10, (2014).

mouse

IHC (frozen)

mouse

IHC (frozen)

Langbein, L., Yoshida, H., Praetzel-Wunder, S., Parry, D. A. & Schweizer, J. The Keratins of the Human Beard Hair Medulla: The Riddle in the Middle. J. Invest. Dermatol. 130, 55â73 (2010).

human

IHC (frozen)

human

IHC (frozen)

Langbein, L. et al. Characterization of a Novel Human Type II Epithelial Keratin K1b, Specifically Expressed in Eccrine Sweat Glands. J. Invest. Dermatol. 125, 428â444 (2005).

human

IHC (frozen)

Herrmann, T. et al. Mice with targeted disruption of the fatty acid transport protein 4 (Fatp 4, Slc27a4) gene show features of lethal restrictive dermopathy. J. Cell Biol. 161, 1105â1115 (2003).

mouse

IHC (paraffin)

human

IHC (paraffin)

Smedts, F. et al. Keratin expression in cervical cancer. Am. J. Pathol. 141, 497â511 (1992).

human

IHC (frozen)

Wetzels, R. H. W. et al. Basal Cell-specific and Hyperproliferation-related Keratins in Human Breast Cancer. Am. J. Pathol. 138, 751â763 (1991).

human

IHC (frozen)

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anti-Keratin K6 mouse monoclonal, KA12, lyophilized, purified

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