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anti-Keratin K6 mouse monoclonal, KA12, supernatant

Excellent marker for non-keratinized squamous epithelia and proliferating cells of epidermis (e.g. within psoriatic lesions).
Cat. No.: 65190
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€143.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone KA12
Immunogen Keratin K6 of human callus cytoskeletal preparation
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.01% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human, mouse

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

Ks6.KA12 represents an excellent marker for non-keratinized squamous epithelia and proliferating cells of epidermis (e.g. within psoriatic lesions).

Polypeptide reacting: Mr 56 000 polypeptide (keratin K6; formerly also designated cytokeratin 6) of human squamous epithelia.

Reactivity on cultured cell lines: A-431, Detroit 562, SCC-12

Tissues and tumors specifically detected: The antibody is suitable for discrimination of keratinizing and non-keratinizing squamous cell carcinoma versus e.g. poorly differentiated adenocarcinoma.

Reference

Reference

PublicationSpeciesApplication
Kist, R. et al. The Formation of Endoderm-Derived Taste Sensory Organs Requires a Pax9-Dependent Expansion of Embryonic Taste Bud Progenitor Cells. PLoS Genet. 10, (2014). mouse IHC (frozen)
Wallace, L., Roberts-Thompson, L. & Reichelt, J. Deletion of K1/K10 does not impair epidermal stratification but affects desmosomal structure and nuclear integrity. J. Cell Sci. 125, 1750–1758 (2012). mouse IHC (frozen)
Langbein, L., Yoshida, H., Praetzel-Wunder, S., Parry, D. A. & Schweizer, J. The Keratins of the Human Beard Hair Medulla: The Riddle in the Middle. J. Invest. Dermatol. 130, 55–73 (2010). human IHC (frozen)
Langbein, L., Eckhart, L., Rogers, M. A., Praetzel-Wunder, S. & Schweizer, J. Against the rules: Human keratin K80 - Two functional alternative splice variants, K80 and K80.1, with special cellular localization in a wide range of epithelia. J. Biol. Chem. 285, 36909–36921 (2010). human IHC (frozen)
Langbein, L. et al. Characterization of a Novel Human Type II Epithelial Keratin K1b, Specifically Expressed in Eccrine Sweat Glands. J. Invest. Dermatol. 125, 428–444 (2005). human IHC (frozen)
Herrmann, T. et al. Mice with targeted disruption of the fatty acid transport protein 4 (Fatp 4, Slc27a4) gene show features of lethal restrictive dermopathy. J. Cell Biol. 161, 1105–1115 (2003). mouse IHC (paraffin)
Langbein, L., Rogers, M. A., Praetzel, S., Winter, H. & Schweizer, J. K6irs1, K6irs2, K6irs3, and K6irs4 represent the inner-root-sheath-specific type II epithelial keratins of the human hair follicle. J. Invest. Dermatol. 120, 512–522 (2003). human WB
Langbein, L. et al. A novel epithelial keratin, hK6irs1, is expressed differentially in all layers of the inner root sheath, including specialized huxley cells (Flügelzellen) of the human hair follicle. J. Invest. Dermatol. 118, 789–799 (2002). human WB
Demirkesen, C., Hoede, N. & Moll, R. Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins. J. Cutan. Pathol. 22, 518–35 (1995). human IHC (paraffin)
Smedts, F. et al. Keratin Expression in Cervical Cancer. Am. J. Pathol. 141, 497–511 (1992). human IHC (frozen)
Wetzels, R. H. W. et al. Basal Cell-specific and Hyperproliferation-related Keratins in Human Breast Cancer. Am. J. Pathol. 138, 751–763 (1991). human IHC (frozen)

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