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Alternative Products
| Cat. No. | Product Name | Format | Quantity | Price |
|---|---|---|---|---|
| 65025 |
anti-Keratin K7 mouse monoclonal, Ks7.18 |
prediluted, purified | 5 mL |
$161.00
Excl. VAT, excl. Shipping Cost
|
| 690025 |
anti-Keratin K7 mouse monoclonal, Ks7.18 |
liquid, purified | 1 mL (50 µg/mL) |
$425.00
Excl. VAT, excl. Shipping Cost
|
| 690025S |
anti-Keratin K7 mouse monoclonal, Ks7.18 |
liquid, purified, small | 0.2 mL (50 µg/mL) |
$170.00
Excl. VAT, excl. Shipping Cost
|
Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG1 |
| Clone | Ks7.18 |
| Immunogen | cytoskeletal proteins from cultured HeLa cells |
| Purification | protein A affinity chromatography |
| Conjugate | unconjugated |
| Formulation | lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4) |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 5000 (IHC, 50 µl/slide) |
| Intended use | Research use only |
| Tested species reactivity | bovine, human, pig, sheep (negative with dog, rabbit, rat, mouse) |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:50 - 1:250 |
| Immunohistochemistry (IHC) - paraffin | 1:50 - 1:250 (protease treatment and/or microwave treatment recommended) |
| Western Blot (WB) | assay dependent |
Background
Details
Ks 7.18 represents an excellent marker for the discrimination of specific subtypes of adenocarcinoma: e.g. adenocarcinoma of pancreas, bile duct carcinoma and transitional carcinoma of bladder are stained, whereas hepatocellular and prostate carcinomas are negative.
Detects specific subtypes of adenocarcinomas: Adenocarcimona of pancreas, gallbladder, lung, cervix; cholangio carcinoma of liver; ductal and lobular carcinoma of breast; carcinomas of ovary; transitional cell carcinoma of bladder; mesothelioma; negative with most cases of hepatocellular carcinoma. In colorectal carcinoma early stages are reported to be negative but advanced stages of tumor development are positive for keratin K7 expression. Occasionally, staining of blood vessel walls, particularly of endothelial cells may be observed.
Reacts with Mr 54,000 polypeptide (keratin K7; formerly also designated cytokeratin 7) of human glandular epithelia.
Reactivity on cultured cell lines: HeLa, RT 112, T24, BT-20, CAMA-1, Detroit 562, (MCF-7 and HT-29 are negative).
Reference
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