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anti-Keratin K7 mouse monoclonal, Ks7.18, prediluted, purified

Detects specific subtypes of adenocarcinoma, e.g. adenocarcinoma of pancreas, bile duct, and transitional carcinoma of the bladder.
Cat. No.: 65025
Quantity:  5 mL

Delivery Time: usually 1-7 working days

Excl. 19% Tax, excl. Shipping Cost

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anti-Keratin K7 mouse monoclonal, Ks7.18

lyophilized, purified 50 µg
Excl. 19% Tax, excl. Shipping Cost

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone Ks7.18
Immunogen cytoskeletal proteins from cultured HeLa cells
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation PBS pH 7.4 with 0.5% BSA and 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, human, pig, sheep (negative with dog, rabbit, rat, mouse)
Intended use Research use only


Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (protease treatment and/ or microwave treatment recommended)
Western Blot (WB) assay dependent



Ks 7.18 represents an excellent marker for the discrimination of specific subtypes of adenocarcinoma: e.g. adenocarcinoma of pancreas, bile duct carcinoma and transitional carcinoma of bladder are stained, whereas hepatocellular and prostate carcinomas are negative.
Detects specific subtypes of adenocarcinomas: Adenocarcimona of pancreas, gallbladder, lung, cervix; cholangio carcinoma of liver; ductal and lobular carcinoma of breast; carcinomas of ovary; transitional cell carcinoma of bladder; mesothelioma; negative with most cases of hepatocellular carcinoma. In colorectal carcinoma early stages are reported to be negative but advanced stages of tumor development are positive for keratin K7 expression. Occasionally, staining of blood vessel walls, particularly of endothelial cells may be observed.
Reacts with Mr 54,000 polypeptide (keratin K7; formerly also designated cytokeratin 7) of human glandular epithelia.

Reactivity on cultured cell lines: HeLa, RT 112, T24, BT-20, CAMA-1, Detroit 562, (MCF-7, HT-29, are negative)


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