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Product description
| Host | guinea pig |
|---|---|
| Antibody Type | polyclonal |
| Immunogen | synthetic peptide of human keratin K73 (formerly also designated K6irs3; YSMLPGGCVTG SGN), coupled to KLH |
| Purification | stabilized antiserum |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide and 0.5% BSA |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 400 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume) |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | human, mouse |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | 1:100 – 1:200 |
| Western Blot (WB) | 1:2,000 |
Background
Details
Reactive polypeptide: The antiserum stains specifically the cuticle layer of the inner root sheath (irs) of the hair follicle. K73 is expressed earlier than K72.
Although there is a considerable sequence homology of keratin K73 polypeptide to keratins K5 and K6, no cross-reactivity was observed with Western blotting and immunofluorescence microscopy.
Reference
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Species
Application
human
IHC (frozen)
