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Keratin K75 (green) and Keratin K1 (red) staining on human hair root. Zoom

Keratin K75 (green) and Keratin K1 (red) staining on human hair root.

anti-Keratin K75 guinea pig polyclonal, serum

The antibody stains specifically the companion layer (innermost layer of outer root sheath) of the hair follicle. No cross-reaction with keratins K5 and K6, although there is considerable sequence homology.
Cat. No.: GP-K6hf
Quantity:  100 µL

Delivery Time: usually 1-7 working days

€260.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host guinea pig
Antibody Type polyclonal
Immunogen synthetic peptide of human type II keratin Human K75 (formerly also designated K6hf) (FTTSGGHSLGAGLGGSGFSATSNRGL - C)
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human, mouse

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:100 - 1:200
Western Blot (WB) 1:2,000

Background

Details

The antiserum stains specifically the companion layer (innermost layer of the outer root sheath) of the hair follicle. Although there is a considerable homology of keratin K75 (K6hf) to keratins K5 and K6, no cross-reactivity was observed, neither in Western blotting nor in immunofluorescence microscopy.

Reference

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