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Product description
| Host | guinea pig |
|---|---|
| Antibody Type | polyclonal |
| Immunogen | synthetic peptide (C-LGGAGSISVSHSGM) of human keratin K76 (formerly also designated keratin 2p), coupled to KLH |
| Purification | stabilized antiserum |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide and 0.5% BSA |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 200 (IHC, 50 µl/slide); ≤ 60 (WB, 5 ml reaction volume) |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | human |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:200 |
| Immunohistochemistry (IHC) - paraffin | 1:100 (microwave treatment recommended) |
| Western Blot (WB) | 1:3,000 |
Background
Details
Specific staining of keratin K76 in middle and upper suprabasal layers of the of palate and gingival epithelia. No cross-reaction with keratin K2! Negative with epithelia of foot sole, scalp, esophagus, duodenum, colon, vagina.
Reactive polypeptide (specificity): Basic human keratin K76 (Mr 64 000)
Positive control: Human palate epithelium
Reference
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