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Product description
Host | guinea pig |
---|---|
Antibody Type | polyclonal |
Immunogen | synthetic peptide (C-LGGAGSISVSHSGM) of human keratin K76 (formerly also designated keratin 2p), coupled to KLH |
Purification | stabilized antiserum |
Conjugate | unconjugated |
Formulation | contains 0.09% sodium azide and 0.5% BSA |
Storage | short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles |
Note | centrifuge prior to opening |
Intended use | Research use only |
Tested species reactivity | human |
Applications
Tested applications | Tested dilutions |
---|---|
Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
Immunohistochemistry (IHC) - frozen | 1:200 |
Immunohistochemistry (IHC) - paraffin | 1:100 (microwave treatment recommended) |
Western Blot (WB) | 1:3,000 |
Background
Details
Specific staining of keratin K76 in middle and upper suprabasal layers of the of palate and gingival epithelia. No cross-reaction with keratin K2! Negative with epithelia of foot sole, scalp, esophagus, duodenum, colon, vagina.
Reactive polypeptide (specificity): Basic human keratin K76 (Mr 64 000)
Positive control: Human palate epithelium
Reference
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