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adult human sole sweat gland (courtesy of L. Langbein) Zoom

adult human sole sweat gland (courtesy of L. Langbein)

anti-Keratin K77 guinea pig polyclonal, serum

K77 (formerly K1b) is closely related to K1 but, in adult humans, is exclusively expressed in luminal duct cells of eccrine sweat glands. The secretory gland is negative as the peripheral cells of the duct. Furthermore, K77 is expressed in suprabasal fetal epidermis, where it appears slightly subsequent to K1. This expression order is also found in conventional and organotypic cultures of human keratinocytes. Ultrastructural investigations show that in contrast to murine K77 intermediate filaments, those of the human ortholog are not attached to desmosomes. After birth, K77 disappears without consequences from human epidermis.
Cat. No.: GP-K77
Quantity:  100 µl

Delivery Time: usually 1-7 working days

€249.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host guinea pig
Antibody Type polyclonal
Immunogen synthetic peptide from human K77(K1b): RVY STS SSA GSG GGS-C
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human (negative with mouse)

Applications

Immunohistochemistry (IHC) - frozen 1:1000
Immunohistochemistry (IHC) - paraffin 1:1000 (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

K77 (formerly K1b) is closely related to K1 but, in adult humans, is exclusively expressed in luminal duct cells of eccrine sweat glands. The secretory gland is negative as the peripheral cells of the duct. Furthermore, K77 is expressed in suprabasal fetal epidermis, where it appears slightly subsequent to K1. This expression order is also found in conventional and organotypic cultures of human keratinocytes. Ultrastructural investigations show that in contrast to murine K77 intermediate filaments, those of the human ortholog are not attached to desmosomes. After birth, K77 disappears without consequences from human epidermis.

Reference

Reference

Langbein, L. et al. New facets of keratin K77: interspecies variations of expression and different intracellular location in embryonic and adult skin of humans and mice. Cell Tissue Res. 354, 793–812 (2013).

Langbein, L. et al. Against the Rules: Human Keratin K80: TWO FUNCTIONAL ALTERNATIVE SPLICE VARIANTS, K80 AND K80.1, WITH SPECIAL CELLULAR LOCALIZATION IN A WIDE RANGE OF EPITHELIA. J. Biol. Chem. 285, 36909–36921 (2010).

Moll, R. et al. The human keratins: biology and pathology. Histochem. Cell Biol. 129, 705–33 (2008).

Langbein, L. et al. Characterization of a novel human type II epithelial keratin K1b, specifically expressed in eccrine sweat glands. J. Invest. Dermatol. 125, 428–444 (2005).

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