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Product description
| Host | guinea pig |
|---|---|
| Antibody Type | polyclonal |
| Immunogen | Synthetic peptide (C - MSIRVTQKSYKVST) of human keratin K8 (formerly also designated cytokeratin 8), coupled to KLH |
| Purification | stabilized antiserum |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide and 0.5% BSA |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 500 (IHC, 50 µl/slide); ≤ 60 (WB, 5 ml reaction volume) |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | bovine, human, mouse, rat |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:50 - 1:250 |
| Immunohistochemistry (IHC) - paraffin | 1:50 - 1:250 (trypsin treatment or microwave treatment recommended) |
| Western Blot (WB) | 1:3,000 |
Background
Details
Specific for keratin K8. Marker for discrimination of simple / non-stratified epithelia from those of stratified origin.
Reactive polypeptide: Basic human keratin K8 (Mr 52 000) of all simple type and complex epithelia.
Positive control: Human intestinal epithelia
Reference
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Species
Application
mouse
IHC-IF (paraffin)
mouse
IHC (paraffin)
