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IHC staining of tumor tissue from mouse CDβGeo cells with anti-Keratin K8/18 (GP11, green), Keratin K5 (Covance, red) and Dapi (blue) (Courtesy of Karen A Dunphy, Veterinary and Animal Sciences, University of Massachusetts) Zoom

IHC staining of tumor tissue from mouse CDβGeo cells with anti-Keratin K8/18 (GP11, green), Keratin K5 (Covance, red) and Dapi (blue) (Courtesy of Karen A Dunphy, Veterinary and Animal Sciences, University of Massachusetts)

anti-Keratin K8/K18 guinea pig polyclonal, serum

The antibody decorates simple and glandular epithelia and neoplasms derived therefrom.
Cat. No.: GP11
Quantity:  100 µL

Delivery Time: usually 1-7 working days

€245.00
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Product description

Host guinea pig
Antibody Type polyclonal
Immunogen Keratin K8/K18 filaments, reconstituted from purified bovine keratins K8 and K18
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, chicken, human, mouse, rat, Syrian hamster, Xenopus laevis

Applications

Tested applications Tested dilutions
ELISA assay dependent
Immunohistochemistry (IHC) - frozen 1:100
Immunohistochemistry (IHC) - paraffin 1:50 (microwave treatment recommended)
Western Blot (WB) 1:2,000

Background

Details

Reactive polypeptide (specificity): Mr 52 500 and 45 500; Keratins K8 and K18 (formerly also designated cytokeratins 8 and 18) of simple type epithelium.
Tumors Specifically Detected: All adenocarcinoma tested, undifferentiated carcinoma, hepatocellular carcinoma, cervix carcinoma

Reference

Reference

PublicationSpeciesApplication
Yang, C. et al. Plakophilin 1-deficient cells upregulate SPOCK1: implications for prostate cancer progression. Tumour Biol. 36, 9567–77 (2015). human IHC (paraffin)
Méndez-Gómez, H. R. et al. Transcytosis in the blood-cerebrospinal fluid barrier of the mouse brain with an engineered receptor/ligand system. Mol. Ther. Methods Clin. Dev. 2, (2015). mouse IHC (free-floating sections)
Song, Y. et al. Carcinoma Initiation via Rb Tumor Suppressor Inactivation: A Versatile Approach to Epithelial Subtype- Dependent Cancer Initiation in Diverse Tissues. PLoS One 8, (2013). mouse IHC (paraffin)
Dunphy, K. A. et al. Oncogenic transformation of mammary epithelial cells by transforming growth factor beta independent of mammary stem cell regulation. Cancer Cell Int. 13, (2013). mouse IHC (paraffin), ICC-IF
König, K. et al. Loss of the Keratin Cytoskeleton Is Not Sufficient to Induce Epithelial Mesenchymal Transition in a Novel KRAS Driven Sporadic Lung Cancer Mouse Model. PLoS One 8, (2013). mouse IHC (paraffin)
Simmons, M. J., Serra, R., Hermance, N. & Kelliher, M. A. NOTCH1 inhibition in vivo results in mammary tumor regression and reduced mammary tumorsphere-forming activity in vitro. Breast Cancer Res. 14, R126 (2012). mouse WB, IHC (paraffin), ICC-IF
Clemons, N. J. et al. Sox9 drives columnar differentiation of esophageal squamous epithelium: a possible role in the pathogenesis of Barrett’s esophagus. AJP Gastrointest. Liver Physiol. 303, G1335–G1346 (2012). mouse WB
Kumar, P. et al. Cooperativity of Rb, Brca1, and p53 in Malignant Breast Cancer Evolution. PLoS Genet. 8, (2012). mouse IHC (paraffin)
Yan, H. et al. Pathways Contributing to Development of Spontaneous Mammary Tumors in BALB/c-Trp53+/− Mice. Am. J. Pathol. 176, 1421–1432 (2010). mouse IHC (paraffin)
Bennecke, M. et al. Ink4a/Arf and Oncogene-Induced Senescence Prevent Tumor Progression during Alternative Colorectal Tumorigenesis. Cancer Cell 18, 135–146 (2010). mouse IHC (paraffin)
Straub, B. K. et al. Lipid droplet-associated PAT-proteins show frequent and differential expression in neoplastic steatogenesis. Mod. Pathol. 23, 480–492 (2010). human IHC (frozen)
Eispert, A.-C. et al. Evidence for distinct populations of human Merkel cells. Histochem. Cell Biol. 132, 83–93 (2009). human IHC (frozen)
Li, Y.-C., Bai, W.-Z., Sakai, K. & Hashikawa, T. Fluorescence and Electron Microscopic Localization of F-actin in the Ependymocytes. J. Histochem. Cytochem. Histochem Cytochem 57, 741–751 (2009). rat IHC (free-floating sections)
Hü Semann, Y. et al. Systemic Spread Is an Early Step in Breast Cancer. Cancer Cell 13, 58–68 (2008). mouse ICC-IF
Straub, B. K., Stoeffel, P., Heid, H., Zimbelmann, R. & Schirmacher, P. Differential pattern of lipid droplet-associated proteins and de novo perilipin expression in hepatocyte steatogenesis. Hepatology 47, 1936–1946 (2008). human, bovine IHC (frozen)
Bultman, S. J. et al. Characterization of mammary tumors from Brg1 heterozygous mice. Oncogene 27, 460–468 (2008). mouse IHC (paraffin)
Herschkowitz, J. I. et al. Identification of conserved gene expression features between murine mammary carcinoma models and human breast tumors. Genome Biol. 8, (2007). human IHC (paraffin)
Mikaelian, I. et al. Expression of Terminal Differentiation Proteins Defines Stages of Mouse Mammary Gland Development. Vet. Pathol. 43, 36–49 (2006). mouse WB, IHC (paraffin)
Moll, I. et al. Human Merkel cells - aspects of cell biology, distribution and functions. Eur. J. Cell Biol. 84, 259–71 (2005). human IHC (frozen)
Galiè, M. et al. Mammary carcinoma provides highly tumourigenic and invasive reactive stromal cells. Carcinogenesis 26, 1868–1878 (2005). mouse IHC (paraffin)
Greten, F. R. et al. Stat3 and NF-kB Activation Prevents Apoptosis in Pancreatic Carcinogenesis. Gastroenterology 123, 2052–2063 (2002). mouse WB
Knapp, A. C. & Franke, W. W. Spontaneous losses of control of cytokeratin gene expression in transformed, non-epithelial human cells occurring at different levels of regulation. Cell 59, 67–79 (1989). human WB, ICC-IF
Franke, W. W. & Moll, R. Cytoskeletal components of lymphoid organs. I. Synthesis of cytokeratins 8 and 18 and desmin in subpopulations of extrafollicular reticulum cells of human lymph nodes, tonsils, and spleen. Differentiation. 36, 145–63 (1987). human IHC (frozen), IEM

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