human kidney (courtesy of J.Heß, University Hospital Heidelberg)

anti-Keratin K8/K18 guinea pig polyclonal, serum

The antibody decorates simple and glandular epithelia and neoplasms derived therefrom.

Cat. No.: GP11
Quantity: 100 µL
No. of experiments: ≤ 200 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume)

Delivery Time: usually 1-7 working days

$375.00

Excl. VAT, excl. Shipping Cost

Qty:

Publication

Species

Application

Hollern, D. et al. A mouse model featuring tissue-specific deletion of p53 and Brca1 gives rise to mammary tumors with genomic and transcriptomic similarities to human basal-like breast cancer. Breast.Cancer.Res.Treat. 174, 143-155 (2019)

mouse

IHC-IF (paraffin)

Komai, M. et al. Pathological Study on Epithelial-Mesenchymal Transition in Silicotic Lung Lesions in Rat. Vet.Sci. 6, (2019)

rat

IHC (paraffin)

Williams, J. et al. Epithelial and stromal circadian clocks are inversely regulated by their mechano-matrix environment. J.Cell.Sci. 131,Â (2018).

mouse

ICC-IF

Linde, N. et al. Macrophages orchestrate breast cancer early dissemination and metastasis. Nat.Commun. 9, 21 (2018).

mouse

IHC (frozen)

Sarrach, S. et al. Spatiotemporal patterning of EpCAM is important for murine embryonic endo- and mesodermal differentiation. Sci.Rep. 8, 1801 (2018).

mouse

IHC (frozen)

Kakehashi, A. et al. Progression of Hepatic Adenoma to Carcinoma in Ogg1 Mutant Mice Induced by Phenobarbital. Oxid.Med.Cell.Longev. 2017, 8541064 (2017).

mouse

IHC (paraffin)

Song, Y. et al. RB inactivation in keratin 18 positive thymic epithelial cells promotes non-cell autonomous T cell hyperproliferation in genetically engineered mice. PLoS One 12, e0171510 (2017)

mouse

ICC-IF

Yang, C. et al. Plakophilin 1-deficient cells upregulate SPOCK1: implications for prostate cancer progression. Tumour Biol. 36, 9567â77 (2015).

human

IHC (paraffin)

MÃ©ndez-GÃ³mez, H. R. et al. Transcytosis in the blood-cerebrospinal fluid barrier of the mouse brain with an engineered receptor/ligand system. Mol. Ther. Methods Clin. Dev. 2, (2015).

mouse

IHC (free floating sections)

Song, Y. et al. Carcinoma Initiation via Rb Tumor Suppressor Inactivation: A Versatile Approach to Epithelial Subtype- Dependent Cancer Initiation in Diverse Tissues. PLoS One 8, (2013).

mouse

IHC (paraffin)

Dunphy, K. A. et al. Oncogenic transformation of mammary epithelial cells by transforming growth factor beta independent of mammary stem cell regulation. Cancer Cell Int. 13, (2013).

mouse

IHC (paraffin)
ICC-IF

KÃ¶nig, K. et al. Loss of the keratin cytoskeleton is not sufficient to induce epithelial mesenchymal transition in a novel KRAS driven sporadic lung cancer mouse model. PLoS One 8, e57996 (2013).

mouse

IHC (paraffin)

Simmons, M. J., Serra, R., Hermance, N. & Kelliher, M. A. NOTCH1 inhibition in vivo results in mammary tumor regression and reduced mammary tumorsphere-forming activity in vitro. Breast Cancer Res. 14, R126 (2012).

mouse

WB
IHC (paraffin)
ICC-IF

Clemons, N. J. et al. Sox9 drives columnar differentiation of esophageal squamous epithelium: a possible role in the pathogenesis of Barrettâs esophagus. AJP Gastrointest. Liver Physiol. 303, G1335âG1346 (2012).

mouse

Kumar, P. et al. Cooperativity of Rb, Brca1, and p53 in Malignant Breast Cancer Evolution. PLoS Genet. 8, (2012).

mouse

IHC (paraffin)

Yan, H. et al. Pathways Contributing to Development of Spontaneous Mammary Tumors in BALB/c-Trp53+/Ã¢Ëâ Mice. Am. J. Pathol. 176, 1421â1432 (2010).

mouse

IHC (paraffin)

Bennecke, M. et al. Ink4a/Arf and Oncogene-Induced Senescence Prevent Tumor Progression during Alternative Colorectal Tumorigenesis. Cancer Cell 18, 135â146 (2010).

mouse

IHC (paraffin)

Straub, B. K. et al. Lipid droplet-associated PAT-proteins show frequent and differential expression in neoplastic steatogenesis. Mod. Pathol. 23, 480â492 (2010).

human

IHC (frozen)

Eispert, A.-C. et al. Evidence for distinct populations of human Merkel cells. Histochem. Cell Biol. 132, 83â93 (2009).

human

IHC (frozen)

Li, Y.-C., Bai, W.-Z., Sakai, K. & Hashikawa, T. Fluorescence and Electron Microscopic Localization of F-actin in the Ependymocytes. J. Histochem. Cytochem. Histochem Cytochem 57, 741â751 (2009).

rat

IHC (free floating sections)

HÃ¼semann, Y. et al. Systemic Spread Is an Early Step in Breast Cancer. Cancer Cell 13, 58â68 (2008).

mouse

ICC-IF

Straub, B. K., Stoeffel, P., Heid, H., Zimbelmann, R. & Schirmacher, P. Differential pattern of lipid droplet-associated proteins and de novo perilipin expression in hepatocyte steatogenesis. Hepatology 47, 1936â1946 (2008).

human

IHC (frozen)

Bultman, S. J. et al. Characterization of mammary tumors from Brg1 heterozygous mice. Oncogene 27, 460â468 (2008).

mouse

IHC (paraffin)

Herschkowitz, J. I. et al. Identification of conserved gene expression features between murine mammary carcinoma models and human breast tumors. Genome Biol. 8, (2007).

human

IHC (paraffin)

Mikaelian, I. et al. Expression of Terminal Differentiation Proteins Defines Stages of Mouse Mammary Gland Development. Vet. Pathol. 43, 36â49 (2006).

mouse

WB
IHC (paraffin)

Moll, I. et al. Human Merkel cells--aspects of cell biology, distribution and functions. Eur. J. Cell Biol. 84, 259â71 (2005).

human

IHC (frozen)

GaliÃ¨, M. et al. Mammary carcinoma provides highly tumourigenic and invasive reactive stromal cells. Carcinogenesis 26, 1868â1878 (2005).

mouse

IHC (paraffin)

Greten, F. R. et al. Stat3 and NF-kB Activation Prevents Apoptosis in Pancreatic Carcinogenesis. Gastroenterology 123, 2052â2063 (2002).

mouse

Knapp, A. C. & Franke, W. W. Spontaneous losses of control of cytokeratin gene expression in transformed, non-epithelial human cells occurring at different levels of regulation. Cell 59, 67â79 (1989).

human

WB
ICC-IF

Franke, W. W. & Moll, R. Cytoskeletal components of lymphoid organs. I. Synthesis of cytokeratins 8 and 18 and desmin in subpopulations of extrafollicular reticulum cells of human lymph nodes, tonsils, and spleen. Differentiation. 36, 145â63 (1987).

human

IHC (frozen)
IEM

Alternative Products

Cat. No.	Product Name	Format	Quantity	Price
GP11S	anti-Keratin K8/K18 guinea pig polyclonal	serum, sample (ready-to-use)	600 µL (ready-to-use)	$65.00 Excl. VAT, excl. Shipping Cost
70020	anti-Keratin sample set		600 µl each antibody	$230.00
70021	anti-skin layer Keratin sample set		600 µl each antibody	$230.00
GP11-WBC	anti-Keratin K8/K18 + positive western blot control		100 µl anti-Keratin K8/K18 + 50 µg western blot control	$405.00

Product description

Host	guinea pig
Antibody Type	polyclonal
Immunogen	Keratin K8/K18 filaments, reconstituted from purified bovine keratins K8 and K18
Purification	stabilized antiserum
Conjugate	unconjugated
Formulation	contains 0.09% sodium azide
Storage	short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments	≤ 200 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume)
Note	centrifuge prior to opening
Intended use	Research use only
Tested species reactivity	bovine, chicken, human, mouse, rat, Syrian hamster

Applications

Tested applications	Tested dilutions
ELISA	assay dependent
Immunohistochemistry (IHC) - frozen	1:100
Immunohistochemistry (IHC) - paraffin	1:50 - 1:100 (microwave treatment recommended)
Western Blot (WB)	1:1,500 - 1:2,500

Background

Details

Reactive polypeptide (specificity): Mr 52 500 and 45 500; Keratins K8 and K18 (formerly also designated cytokeratins 8 and 18) of simple type epithelium.
Tumors Specifically Detected: All adenocarcinoma tested, undifferentiated carcinoma, hepatocellular carcinoma, cervix carcinoma

Positive western blot control lysate available:
MCF7 western blot control, Cat. No. 64003
MCF7 western blot control, large, Cat. No. 64003L

Reference

Publication

Species

Application

mouse

IHC-IF (paraffin)

Komai, M. et al. Pathological Study on Epithelial-Mesenchymal Transition in Silicotic Lung Lesions in Rat. Vet.Sci. 6, (2019)

rat

IHC (paraffin)

Williams, J. et al. Epithelial and stromal circadian clocks are inversely regulated by their mechano-matrix environment. J.Cell.Sci. 131,Â (2018).

mouse

ICC-IF

Linde, N. et al. Macrophages orchestrate breast cancer early dissemination and metastasis. Nat.Commun. 9, 21 (2018).

mouse

IHC (frozen)

Sarrach, S. et al. Spatiotemporal patterning of EpCAM is important for murine embryonic endo- and mesodermal differentiation. Sci.Rep. 8, 1801 (2018).

mouse

IHC (frozen)

Kakehashi, A. et al. Progression of Hepatic Adenoma to Carcinoma in Ogg1 Mutant Mice Induced by Phenobarbital. Oxid.Med.Cell.Longev. 2017, 8541064 (2017).

mouse

IHC (paraffin)

Song, Y. et al. RB inactivation in keratin 18 positive thymic epithelial cells promotes non-cell autonomous T cell hyperproliferation in genetically engineered mice. PLoS One 12, e0171510 (2017)

mouse

ICC-IF

Yang, C. et al. Plakophilin 1-deficient cells upregulate SPOCK1: implications for prostate cancer progression. Tumour Biol. 36, 9567â77 (2015).

human

IHC (paraffin)

MÃ©ndez-GÃ³mez, H. R. et al. Transcytosis in the blood-cerebrospinal fluid barrier of the mouse brain with an engineered receptor/ligand system. Mol. Ther. Methods Clin. Dev. 2, (2015).

mouse

IHC (free floating sections)

Song, Y. et al. Carcinoma Initiation via Rb Tumor Suppressor Inactivation: A Versatile Approach to Epithelial Subtype- Dependent Cancer Initiation in Diverse Tissues. PLoS One 8, (2013).

mouse

IHC (paraffin)

Dunphy, K. A. et al. Oncogenic transformation of mammary epithelial cells by transforming growth factor beta independent of mammary stem cell regulation. Cancer Cell Int. 13, (2013).

mouse

IHC (paraffin)
ICC-IF

KÃ¶nig, K. et al. Loss of the keratin cytoskeleton is not sufficient to induce epithelial mesenchymal transition in a novel KRAS driven sporadic lung cancer mouse model. PLoS One 8, e57996 (2013).

mouse

IHC (paraffin)

mouse

WB
IHC (paraffin)
ICC-IF

mouse

Kumar, P. et al. Cooperativity of Rb, Brca1, and p53 in Malignant Breast Cancer Evolution. PLoS Genet. 8, (2012).

mouse

IHC (paraffin)

Yan, H. et al. Pathways Contributing to Development of Spontaneous Mammary Tumors in BALB/c-Trp53+/Ã¢Ëâ Mice. Am. J. Pathol. 176, 1421â1432 (2010).

mouse

IHC (paraffin)

Bennecke, M. et al. Ink4a/Arf and Oncogene-Induced Senescence Prevent Tumor Progression during Alternative Colorectal Tumorigenesis. Cancer Cell 18, 135â146 (2010).

mouse

IHC (paraffin)

Straub, B. K. et al. Lipid droplet-associated PAT-proteins show frequent and differential expression in neoplastic steatogenesis. Mod. Pathol. 23, 480â492 (2010).

human

IHC (frozen)

Eispert, A.-C. et al. Evidence for distinct populations of human Merkel cells. Histochem. Cell Biol. 132, 83â93 (2009).

human

IHC (frozen)

Li, Y.-C., Bai, W.-Z., Sakai, K. & Hashikawa, T. Fluorescence and Electron Microscopic Localization of F-actin in the Ependymocytes. J. Histochem. Cytochem. Histochem Cytochem 57, 741â751 (2009).

rat

IHC (free floating sections)

HÃ¼semann, Y. et al. Systemic Spread Is an Early Step in Breast Cancer. Cancer Cell 13, 58â68 (2008).

mouse

ICC-IF

human

IHC (frozen)

Bultman, S. J. et al. Characterization of mammary tumors from Brg1 heterozygous mice. Oncogene 27, 460â468 (2008).

mouse

IHC (paraffin)

Herschkowitz, J. I. et al. Identification of conserved gene expression features between murine mammary carcinoma models and human breast tumors. Genome Biol. 8, (2007).

human

IHC (paraffin)

Mikaelian, I. et al. Expression of Terminal Differentiation Proteins Defines Stages of Mouse Mammary Gland Development. Vet. Pathol. 43, 36â49 (2006).

mouse

WB
IHC (paraffin)

Moll, I. et al. Human Merkel cells--aspects of cell biology, distribution and functions. Eur. J. Cell Biol. 84, 259â71 (2005).

human

IHC (frozen)

GaliÃ¨, M. et al. Mammary carcinoma provides highly tumourigenic and invasive reactive stromal cells. Carcinogenesis 26, 1868â1878 (2005).

mouse

IHC (paraffin)

Greten, F. R. et al. Stat3 and NF-kB Activation Prevents Apoptosis in Pancreatic Carcinogenesis. Gastroenterology 123, 2052â2063 (2002).

mouse

human

WB
ICC-IF

human

IHC (frozen)
IEM

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anti-Keratin K8/K18 guinea pig polyclonal, serum

Alternative Products