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Alternative Products
Cat. No. | Product Name | Format | Quantity | Price |
---|---|---|---|---|
GP11S |
anti-Keratin K8/K18 guinea pig polyclonal |
serum, sample (ready-to-use) | 600 µL (ready-to-use) |
$65.00
Excl. VAT, excl. Shipping Cost
|
70020 |
anti-Keratin sample set |
600 µl each antibody |
$230.00
|
|
70021 |
anti-skin layer Keratin sample set |
600 µl each antibody |
$230.00
|
|
GP11-WBC |
anti-Keratin K8/K18 + positive western blot control |
100 µl anti-Keratin K8/K18 + 50 µg western blot control |
$405.00
|
Product description
Host | guinea pig |
---|---|
Antibody Type | polyclonal |
Immunogen | Keratin K8/K18 filaments, reconstituted from purified bovine keratins K8 and K18 |
Purification | stabilized antiserum |
Conjugate | unconjugated |
Formulation | contains 0.09% sodium azide |
Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
No. of experiments | ≤ 200 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume) |
Note | centrifuge prior to opening |
Intended use | Research use only |
Tested species reactivity | bovine, chicken, human, mouse, rat, Syrian hamster |
Applications
Tested applications | Tested dilutions |
---|---|
ELISA | assay dependent |
Immunohistochemistry (IHC) - frozen | 1:100 |
Immunohistochemistry (IHC) - paraffin | 1:50 - 1:100 (microwave treatment recommended) |
Western Blot (WB) | 1:1,500 - 1:2,500 |
Background
Details
Reactive polypeptide (specificity): Mr 52 500 and 45 500; Keratins K8 and K18 (formerly also designated cytokeratins 8 and 18) of simple type epithelium.
Tumors Specifically Detected: All adenocarcinoma tested, undifferentiated carcinoma, hepatocellular carcinoma, cervix carcinoma
Positive western blot control lysate available:
MCF7 western blot control, Cat. No. 64003
MCF7 western blot control, large, Cat. No. 64003L
Reference
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Species
Application
rat
IHC (paraffin)
mouse
IHC (frozen)
mouse
IHC (frozen)
mouse
IHC (paraffin)
human
IHC (paraffin)
mouse
IHC (free floating sections)
mouse
IHC (paraffin)
mouse
IHC (paraffin)
ICC-IF
ICC-IF
mouse
WB
IHC (paraffin)
ICC-IF
IHC (paraffin)
ICC-IF
mouse
IHC (paraffin)
mouse
IHC (paraffin)
mouse
IHC (paraffin)
human
IHC (frozen)
human
IHC (frozen)
rat
IHC (free floating sections)
mouse
ICC-IF
mouse
IHC (paraffin)
human
IHC (paraffin)
mouse
WB
IHC (paraffin)
IHC (paraffin)
human
IHC (frozen)
mouse
IHC (paraffin)