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human kidney (courtesy of J.Heß, University Hospital Heidelberg) Zoom

human kidney (courtesy of J.Heß, University Hospital Heidelberg)

anti-Keratin K8/K18 guinea pig polyclonal, serum

The antibody decorates simple and glandular epithelia and neoplasms derived therefrom.
application_confirmed highly_published external_validation
Cat. No.: GP11
Quantity:  100 µL

Delivery Time: usually 1-7 working days

$375.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

Alternative Products

Cat. No. Product Name Format Quantity Price
GP11S

anti-Keratin K8/K18 guinea pig polyclonal

serum, sample (ready-to-use) 600 µL (ready-to-use)
$65.00
Excl. 19% Tax, excl. Shipping Cost
70020

anti-Keratin sample set

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GP11-WBC

anti-Keratin K8/K18 + positive western blot control

100 µl anti-Keratin K8/K18 + 50 µg western blot control
$405.00

Product description

Host guinea pig
Antibody Type polyclonal
Immunogen Keratin K8/K18 filaments, reconstituted from purified bovine keratins K8 and K18
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity bovine, chicken, human, mouse, rat, Syrian hamster

Applications

Tested applications Tested dilutions
ELISA assay dependent
Immunohistochemistry (IHC) - frozen 1:100
Immunohistochemistry (IHC) - paraffin 1:50 - 1:100 (microwave treatment recommended)
Western Blot (WB) 1:1,500 - 1:2,500

Background

Details

Reactive polypeptide (specificity): Mr 52 500 and 45 500; Keratins K8 and K18 (formerly also designated cytokeratins 8 and 18) of simple type epithelium.
Tumors Specifically Detected: All adenocarcinoma tested, undifferentiated carcinoma, hepatocellular carcinoma, cervix carcinoma

Positive western blot control lysate available:
MCF7 western blot control, Cat. No. 64003
MCF7 western blot control, large, Cat. No. 64003L

Reference

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