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anti-Keratin K8/K18 guinea pig polyclonal, serum

The antibody decorates simple and glandular epithelia and neoplasms derived therefrom.
application_confirmed highly_published external_validation
Cat. No.: GP11
Quantity:  100 µL
No. of experiments:  ≤ 200 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume)

Delivery Time: usually 1-7 working days

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Product description

Host guinea pig
Antibody Type polyclonal
Immunogen Keratin K8/K18 filaments, reconstituted from purified bovine keratins K8 and K18
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 200 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume)
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity bovine, chicken, human, mouse, rat, Syrian hamster


Tested applications Tested dilutions
ELISA assay dependent
Immunohistochemistry (IHC) - frozen 1:100
Immunohistochemistry (IHC) - paraffin 1:50 - 1:100 (microwave treatment recommended)
Western Blot (WB) 1:1,500 - 1:2,500



Reactive polypeptide (specificity): Mr 52 500 and 45 500; Keratins K8 and K18 (formerly also designated cytokeratins 8 and 18) of simple type epithelium.
Tumors Specifically Detected: All adenocarcinoma tested, undifferentiated carcinoma, hepatocellular carcinoma, cervix carcinoma

Positive western blot control lysate available:
MCF7 western blot control, Cat. No. 64003
MCF7 western blot control, large, Cat. No. 64003L


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