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Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG1 |
| Clone | Ks8-17.2 |
| Immunogen | Cytoskeletal proteins from human tumor cell line MCF-7 |
| Purification | hybridoma cell culture supernatant |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 100 (IHC, 50 µl/slide) |
| Intended use | Research use only |
| Tested species reactivity | bovine, hamster, human, rat |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
| Immunohistochemistry (IHC) - frozen | ready-to-use |
| Western Blot (WB) | assay dependent |
Background
Details
Ks 8-17.2 represents an excellent marker to discriminate simple epithelia from those of different origin.
Tumors specifically detected: All adeno-CA tested; undifferentiated CA; cervix CA; hepatocellular CA.
Polypeptide reacting: Mr 52 500 polypeptide (keratin K8; formerly also designated cytokeratin 8) of human epithelia.
Reactivity on cultured cell lines: MCF-7, HeLa, A-431, PLC, Detroit 562.
Reference
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