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IHC of human liver (courtesy of J.Heß, University Hospital Heidelberg) Zoom

IHC of human liver (courtesy of J.Heß, University Hospital Heidelberg)

anti-Keratin K8 mouse monoclonal, Ks8.7, lyophilized, purified

Recognizes all simple-type epithelia and neoplasms derived therefrom. Stains all adenocarcinomas tested and undifferentiated carcinomas.
application_confirmed highly_published external_validation validated_by_epitope_mapping
Cat. No.: 61038
Quantity:  50 µg

Delivery Time: usually 1-7 working days

€232.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone Ks8.7
Immunogen cytoskeletal proteins from cultured HeLa cells
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Intended use Research use only
Tested species reactivity hamster, human (negative with bovine, pig)

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:50 - 1:100
Immunohistochemistry (IHC) - paraffin 1:50 - 1:100 (microwave treatment recommended)
Western Blot (WB) 1:50 - 1:500

Background

Details

Ks 8.7 represents an excellent marker to discriminate simple epithelia from those of different origin.

Polypeptide reacting: Mr 52 500 polypeptide (keratin K8; formerly also designated cytokeratin 8) of human epithelia.

Reactivity on cultured cell lines: MCF-7

Tumors specifically detected: All adenocarcinoma tested; undifferentiated carcinoma; cervix carcinoma; hepatocellular carcinoma

Reference

Reference

PublicationSpeciesApplication
Larribere, L. et al. An RNAi Screen Reveals an Essential Role for HIPK4 in Human Skin Epithelial Differentiation from iPSCs. Stem.Cell.Reports. 9, 1234-1245 (2017). human IHC-IF (paraffin)
Bitam, S. et al. An unexpected effect of TNF-α on F508del-CFTR maturation and function. F1000 Res. 4, (2015). human ICC-IF
Zatloukal, B. et al. Sensitivity and Specificity of In situ Proximity Ligation for Protein Interaction Analysis in a Model of Steatohepatitis with Mallory-Denk Bodies. PLoS One 9, (2014). mouse IHC (frozen)
Ito, K. et al. Loss of Runx3 Is a Key Event in Inducing Precancerous State of the Stomach. Gastroenterology 140, 1536–1546.e8 (2011). mouse IHC (paraffin)
Plum, W. et al. Lack of Glycoprotein 130/Signal Transducer and Activator of Transcription 3-Mediated Signaling in Hepatocytes Enhances Chronic Liver Injury and Fibrosis Progression in a Model of Sclerosing Cholangitis. Am. J. Pathol. 176, 2236–2246 (2010). mouse IHC (frozen)
Langbein, L. et al. Characterization of a Novel Human Type II Epithelial Keratin K1b, Specifically Expressed in Eccrine Sweat Glands. J. Invest. Dermatol. 125, 428–444 (2005). human IHC (frozen)
Zatloukal, K. et al. p62 Is a Common Component of Cytoplasmic Inclusions in Protein Aggregation Diseases. Am. J. Pathol. 160, 255–263 (2002). human ICC-IF
Stumptner, C., Fuchsbichler, A., Heid, H., Zatloukal, K. & Denk, H. Mallory body—A disease-associated type of sequestosome. Hepatology 35, 1053–1062 (2002). human, mouse IHC (frozen), IEM

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