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upcyte hepatocytes, dilution 1:50 (courtesy of upcyte technologies GmbH) Zoom

upcyte hepatocytes, dilution 1:50 (courtesy of upcyte technologies GmbH)

anti-Keratin K8 mouse monoclonal, Ks8.7, lyophilized, purified

Recognizes all simple-type epithelia and neoplasms derived therefrom. Stains all adenocarcinomas tested and undifferentiated carcinomas.
Cat. No.: 61038
Quantity:  50 µg

Delivery Time: usually 1-7 working days

€201.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone Ks8.7
Immunogen cytoskeletal proteins from cultured HeLa cells
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity hamster, human, monodelphis, mouse, rat, tupaia (negative with bovine, pig)

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:10
Immunohistochemistry (IHC) - paraffin 1:10 (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

Ks 8.7 represents an excellent marker to discriminate simple epithelia from those of different origin.

Polypeptide reacting: Mr 52 500 polypeptide (keratin K8; formerly also designated cytokeratin 8) of human epithelia.

Reactivity on cultured cell lines: MCF-7, RT 112, PLC

Tumors specifically detected: All adenocarcinoma tested; undifferentiated carcinoma; cervix carcinoma; hepatocellular carcinoma

Reference

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