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upcyte hepatocytes, dilution 1:50 (courtesy of upcyte technologies GmbH) Zoom

upcyte hepatocytes, dilution 1:50 (courtesy of upcyte technologies GmbH)

anti-Keratin K8 mouse monoclonal, Ks8.7, lyophilized, purified

Recognizes all simple-type epithelia and neoplasms derived therefrom. Stains all adenocarcinomas tested and undifferentiated carcinomas.
Cat. No.: 61038
Quantity:  50 µg

Delivery Time: usually 1-7 working days

€201.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone Ks8.7
Immunogen cytoskeletal proteins from cultured HeLa cells
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity hamster, human, monodelphis, mouse, rat, tupaia (not with bovine, pig)

Applications

Immunohistochemistry (IHC) - frozen 1:10
Immunohistochemistry (IHC) - paraffin 1:10 (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

Ks 8.7 represents an excellent marker to discriminate simple epithelia from those of different origin. Polypeptide reacting: Mr 52 500 polypeptide (keratin K8; formerly also designated cytokeratin 8) of human epithelia. Reactivity on cultured cell lines: MCF-7, RT 112, PLC Tumors specifically detected: All adenocarcinoma tested; undifferentiated carcinoma; cervix carcinoma; hepatocellular carcinoma

Reference

Reference

  1. Bitam, S. et al. An unexpected effect of TNF-α on F508del-CFTR maturation and function. F1000 Res. 4, (2015).
  2. Zatloukal, B. et al. Sensitivity and Specificity of In situ Proximity Ligation for Protein Interaction Analysis in a Model of Steatohepatitis with Mallory-Denk Bodies. PLoS One 9, (2014).
  3. Ito, K. et al. Loss of Runx3 Is a Key Event in Inducing Precancerous State of the Stomach. Gastroenterology 140, 1536–1546.e8 (2011).
  4. Plum, W. et al. Lack of Glycoprotein 130/Signal Transducer and Activator of Transcription 3-Mediated Signaling in Hepatocytes Enhances Chronic Liver Injury and Fibrosis Progression in a Model of Sclerosing Cholangitis. Am. J. Pathol. 176, 2236–2246 (2010).
  5. Fukaya, M. et al. Hedgehog Signal Activation in Gastric Pit Cell and in Diffuse-Type Gastric Cancer. Gastroenterology 131, 14–29 (2006).
  6. Stumptner, C., Fuchsbichler, A., Heid, H., Zatloukal, K. & Denk, H. Mallory body—A disease-associated type of sequestosome. Hepatology 35, 1053–1062 (2002).
  7. Gomi, H. et al. Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prions. Neuron. 14, 29–41 (1995).
  8. Bártek, J. et al. A series of 14 new monoclonal antibodies to keratins: Characterization and value in diagnostic histopathology. J. Pathol. 164, 215–224 (1991).
  9. Osborn, M., van Lessen, G., Weber, K., Klöppel, G. & Altmannsberger, M. Differential diagnosis of gastrointestinal carcinomas by using monoclonal antibodies specific for individual keratin polypeptides. Lab. Invest. 55, 497–504 (1986).
  10. Moll, R., Franke, W. W., Schiller, D. L., Geiger, B. & Krepler, R. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31, 11–24 (1982).

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