IHC of human liver (courtesy of J.Heß, University Hospital Heidelberg)

anti-Keratin K8 mouse monoclonal, Ks8.7, lyophilized, purified

Recognizes all simple-type epithelia and neoplasms derived therefrom. Stains all adenocarcinomas tested and undifferentiated carcinomas.

Cat. No.: 61038
Quantity: 50 µg
No. of experiments: ≤ 2000 (IHC, 50 µl/slide); ≤ 100 (WB, 5 ml reaction volume)

Delivery Time: usually 1-7 working days

$319.00

Excl. VAT, excl. Shipping Cost

Qty:

Publication

Species

Application

Larribere, L. et al. An RNAi Screen Reveals an Essential Role for HIPK4 in Human Skin Epithelial Differentiation from iPSCs. Stem.Cell.Reports. 9, 1234-1245 (2017).

human

IHC-IF (paraffin)

Bitam, S. et al. An unexpected effect of TNF-? on F508del-CFTR maturation and function. F1000 Res. 4, (2015).

human

ICC-IF

Zatloukal, B. et al. Sensitivity and specificity of in situ proximity ligation for protein interaction analysis in a model of steatohepatitis with mallory-denk bodies. PLoS One 9, (2014).

mouse

IHC (frozen)

Ito, K. et al. Loss of Runx3 Is a Key Event in Inducing Precancerous State of the Stomach. Gastroenterology 140, 1536â1546.e8 (2011).

mouse

IHC (paraffin)

Plum, W. et al. Lack of Glycoprotein 130/Signal Transducer and Activator of Transcription 3-Mediated Signaling in Hepatocytes Enhances Chronic Liver Injury and Fibrosis Progression in a Model of Sclerosing Cholangitis. Am. J. Pathol. 176, 2236â2246 (2010)

mouse

IHC (frozen)

Langbein, L. et al. Characterization of a Novel Human Type II Epithelial Keratin K1b, Specifically Expressed in Eccrine Sweat Glands. J. Invest. Dermatol. 125, 428â444 (2005).

human

IHC (frozen)

Zatloukal, K. et al. p62 Is a Common Component of Cytoplasmic Inclusions in Protein Aggregation Diseases. Am. J. Pathol. 160, 255â262 (2002).

human

ICC-IF

Stumptner, C. Mallory bodyâA disease-associated type of sequestosome. Hepatology 35, 1053â1062 (2002).

human
mouse

IHC (frozen)
IEM

Alternative Products

Cat. No.	Product Name	Format	Quantity	Price
19038	anti-Keratin K8 mouse monoclonal, Ks8.7	prediluted, purified	1 mL	$272.00 Excl. VAT, excl. Shipping Cost
61038S	anti-Keratin K8 mouse monoclonal, Ks8.7	purified, sample (ready-to-use)	600 µL (ready-to-use)	$65.00 Excl. VAT, excl. Shipping Cost
70023	anti-Keratin K8/K18 sample set		600 µl each antibody	$105.00
61038-WBC	anti-Keratin K8, Ks8.7 + positive western blot control		50 µg anti-Keratin K8 + 50 µg western blot control	$260.00
690038	anti-Keratin K8 mouse monoclonal, Ks8.7	liquid, purified	1 mL (50 µg/mL)	$425.00 Excl. VAT, excl. Shipping Cost
690038S	anti-Keratin K8 mouse monoclonal, Ks8.7	liquid, purified, small	0.2 mL (50 µg/mL)	$170.00 Excl. VAT, excl. Shipping Cost

Product description

Host	mouse
Antibody Type	monoclonal
Isotype	IgG1
Clone	Ks8.7
Immunogen	cytoskeletal proteins from cultured HeLa cells
Purification	protein A affinity chromatography
Conjugate	unconjugated
Formulation	lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage	short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments	≤ 2000 (IHC, 50 µl/slide); ≤ 100 (WB, 5 ml reaction volume)
Intended use	Research use only
Tested species reactivity	hamster, human (negative with bovine, pig)

Applications

Tested applications	Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF)	assay dependent
Immunohistochemistry (IHC) - frozen	1:50 - 1:100
Immunohistochemistry (IHC) - paraffin	1:50 - 1:100 (microwave treatment recommended)
Western Blot (WB)	1:50 - 1:500

Background

Details

Ks 8.7 represents an excellent marker to discriminate simple epithelia from those of different origin.

Polypeptide reacting: Mr 52 500 polypeptide (keratin K8; formerly also designated cytokeratin 8) of human epithelia.

Reactivity on cultured cell lines: MCF-7

Tumors specifically detected: All adenocarcinoma tested; undifferentiated carcinoma; cervix carcinoma; hepatocellular carcinoma

Reference

Publication

Species

Application

Larribere, L. et al. An RNAi Screen Reveals an Essential Role for HIPK4 in Human Skin Epithelial Differentiation from iPSCs. Stem.Cell.Reports. 9, 1234-1245 (2017).

human

IHC-IF (paraffin)

Bitam, S. et al. An unexpected effect of TNF-? on F508del-CFTR maturation and function. F1000 Res. 4, (2015).

human

ICC-IF

Zatloukal, B. et al. Sensitivity and specificity of in situ proximity ligation for protein interaction analysis in a model of steatohepatitis with mallory-denk bodies. PLoS One 9, (2014).

mouse

IHC (frozen)

Ito, K. et al. Loss of Runx3 Is a Key Event in Inducing Precancerous State of the Stomach. Gastroenterology 140, 1536â1546.e8 (2011).

mouse

IHC (paraffin)

mouse

IHC (frozen)

Langbein, L. et al. Characterization of a Novel Human Type II Epithelial Keratin K1b, Specifically Expressed in Eccrine Sweat Glands. J. Invest. Dermatol. 125, 428â444 (2005).

human

IHC (frozen)

Zatloukal, K. et al. p62 Is a Common Component of Cytoplasmic Inclusions in Protein Aggregation Diseases. Am. J. Pathol. 160, 255â262 (2002).

human

ICC-IF

Stumptner, C. Mallory bodyâA disease-associated type of sequestosome. Hepatology 35, 1053â1062 (2002).

human
mouse

IHC (frozen)
IEM

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anti-Keratin K8 mouse monoclonal, Ks8.7, lyophilized, purified

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