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Alternative Products
Cat. No. | Product Name | Format | Quantity | Price |
---|---|---|---|---|
19038 |
anti-Keratin K8 mouse monoclonal, Ks8.7 |
prediluted, purified | 1 mL |
$272.00
Excl. VAT, excl. Shipping Cost
|
61038S |
anti-Keratin K8 mouse monoclonal, Ks8.7 |
purified, sample (ready-to-use) | 600 µL (ready-to-use) |
$65.00
Excl. VAT, excl. Shipping Cost
|
70023 |
anti-Keratin K8/K18 sample set |
600 µl each antibody |
$105.00
|
|
61038-WBC |
anti-Keratin K8, Ks8.7 + positive western blot control |
50 µg anti-Keratin K8 + 50 µg western blot control |
$301.00
|
|
690038 |
anti-Keratin K8 mouse monoclonal, Ks8.7 |
liquid, purified | 1 mL (50 µg/mL) |
$425.00
Excl. VAT, excl. Shipping Cost
|
690038S |
anti-Keratin K8 mouse monoclonal, Ks8.7 |
liquid, purified, small | 0.2 mL (50 µg/mL) |
$170.00
Excl. VAT, excl. Shipping Cost
|
Product description
Host | mouse |
---|---|
Antibody Type | monoclonal |
Isotype | IgG1 |
Clone | Ks8.7 |
Immunogen | cytoskeletal proteins from cultured HeLa cells |
Purification | protein A affinity chromatography |
Conjugate | unconjugated |
Formulation | lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4) |
Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
No. of experiments | ≤ 2000 (IHC, 50 µl/slide); ≤ 100 (WB, 5 ml reaction volume) |
Intended use | Research use only |
Tested species reactivity | hamster, human (negative with bovine, pig) |
Applications
Tested applications | Tested dilutions |
---|---|
Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
Immunohistochemistry (IHC) - frozen | 1:50 - 1:100 |
Immunohistochemistry (IHC) - paraffin | 1:50 - 1:100 (microwave treatment recommended) |
Western Blot (WB) | 1:50 - 1:500 |
Background
Details
Ks 8.7 represents an excellent marker to discriminate simple epithelia from those of different origin.
Polypeptide reacting: Mr 52 500 polypeptide (keratin K8; formerly also designated cytokeratin 8) of human epithelia.
Reactivity on cultured cell lines: MCF-7
Tumors specifically detected: All adenocarcinoma tested; undifferentiated carcinoma; cervix carcinoma; hepatocellular carcinoma
Reference
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IHC-IF (paraffin)
human
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mouse
IHC (paraffin)
human
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mouse
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