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anti-Keratin K8 mouse monoclonal, M20, supernatant

Cytokeratin 8 is present in simple epithelia, in glandular epithelium and in most tumors derived therefrom.
Cat. No.: 10526
Quantity:  1 mL

Delivery Time: usually 1-7 working days

€228.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone M20
Immunogen human breast carcinoma cell line
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human, mammals (negative with mouse)

Applications

Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:5 - 1:10
Immunohistochemistry (IHC) - frozen 1:5 - 1:10

Background

Details

In immunoblotting, the antibody is directed against the 52.2 kD keratin protein K8 (formerly also designated cytokeratin 8). M20 is exclusively reactive with keratin K8, present in simple type epithelia, in glandular epithelium and in most tumors derived therefrom. Epitope has been mapped to aa 353-367 on alpha helical rod domain (see Waseem et al., 2004)

Recommended for positive control: Glandular epithelia

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