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anti-Keratin K9 guinea pig polyclonal, serum

Excellent marker to study palmoplantar epidermal distribution and differentiation.
Cat. No.: GP-CK9
Quantity:  100 µL

Delivery Time: usually 1-7 working days

€249.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host guinea pig
Antibody Type polyclonal
Immunogen Synthetic peptide of human keratin K9 (corresponding to aa 450-477), coupled to KLH
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, human, mouse, rat

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:100 - 1:200
Immunohistochemistry (IHC) - paraffin 1:50 (microwave treatment recommended)
Western Blot (WB) 1:5,000 - 1:10,000

Background

Details

The antiserum represents an excellent marker to study palmoplantar epidermal distribution and differentiation, specifically reactive in the middle/upper suprabasal layers (stratum spinosum/ granulosum) of the epidermis of palm and sole. K9 can be detected in primary cultures of palmoplantar keratinocytes when they shift to differentiation-promoting conditions and grow stratified (upper cells). K9 has not been found in normal, i.e. non-pathogenic, non-ridged epidermis, beside some minor cells surrounding the acrosyringeal ducts. No labelling has been found in epithelial cells of other stratified epithelia such as oesophagus or complex epithelia (e.g. urothelium) or in ductal or simple epithelia.
Negative tissues include: muscle, liver and duodenum. (see references listed below).
Reactive polypeptide: Acidic human keratin K9 (MW 62,129; formerly also designated cytokeratin 9), expressed in the palmoplantar epidermis

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