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Keratin K9 staining on human tissue (courtesy of The Human Protein Atlas) Zoom

Keratin K9 staining on human tissue (courtesy of The Human Protein Atlas)

anti-Keratin K9 mouse monoclonal, Ks9.70 + Ks9.216, supernatant

Excellent marker to study palmoplantar epidermal differentiation. Reactive with keratinocytes in the upper suprabasal layers of palm and sole. New tool to characterize primary cultures for application in burn treatment.
Cat. No.: 651104
Quantity:  1 mL

Delivery Time: usually 1-7 working days

€196.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1 and IgG3
Clone Ks9.70 + Ks9.216
Immunogen synthetic peptides [(1) amino acids pos. 450-477 and (2) N-terminal amino acids nos. 4-28] of human keratin K9 (MW 62,129; formerly also designated cytokeratin 9)
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human (negative with mouse)

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:10
Immunohistochemistry (IHC) - frozen 1:20
Immunohistochemistry (IHC) - paraffin 1:20 (protease treatment and/or microwave treatment recommended)
Western Blot (WB) 1:25 - 1:300

Background

Details

The antibody reagent represents an excellent marker to study palmoplantar epidermal distribution and differentiation. Specifically reactive in the middle/upper suprabasal layers (stratum spinosum/ granulosum) of the epidermis of palm and sole. K9 can be detected in primary cultures of palmoplantar keratinocytes when they shift to differentiation-promoting conditions and grow stratified (upper cells). K9 has not been found in normal, i.e. non-pathogenic, non-ridged epidermis, beside some minor cells surrounding the acrosyringeal ducts. No labelling has been found in epithelial cells of other stratified epithelia such as oesophagus or complex epithelia (e.g. urothelium) or in ductal or simple epithelia. Negative tissues include: muscle, liver and duodenum.

The antibody cocktail is an excellent new tool to characterize primary cultures of keratinocytes/skin transplants for application in burn treatment.

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