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mouse tongue (courtesy of J.Heß, University Hospital Heidelberg) Zoom

mouse tongue (courtesy of J.Heß, University Hospital Heidelberg)

anti-Keratin Pan mouse monoclonal, Lu-5, purified

Reacts with all types of epithelia and epithelium-derived neoplasms.
highly_published external_validation
Cat. No.: 610145
Quantity:  50 µg (lyoph.)

Delivery Time: usually 1-7 working days

€196.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone Lu-5
Immunogen cells of lung cancer cell line A549 and A2182
Purification affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Intended use Research use only
Tested species reactivity bovine, chicken, human, mouse, Pleurodelis, rat, snake, xenopus

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen assay dependent
Immunohistochemistry (IHC) - paraffin 1:10 - 1:20 (protease treatment and/or microwave treatment recommended)
Western Blot (WB) not applicable

Background

Details

Lu-5 represents an excellent marker for all types of epithelia. Ideal marker for immunolocalization studies on frozen and paraffin embedded tissue. Useful for distinguishing carcinomas from non-epithelial tumors.
Tumors specifically detected: all epithelium-derived neoplasms.

Polypeptide reacting: Most keratins present in human epithelia (including keratins – formerly also designated cytokeratins -K1, K5, K6, K14, K18, K19) and most acidic (K31 – KK38) and basic (K81 – K86) hair keratins from human hair follicle. The antibody specifically detects a conformational epitope common to most keratins, but not to other intermediate filament polypeptides.

Reactivity on cultured cell lines: MCF-7, PLC, A-431, HeLa; PtK2, A6 (XLKE).

Reference

Reference

PublicationSpeciesApplication
Rickelt, S. Plakophilin-2: a cell-cell adhesion plaque molecule of selective and fundamental importance in cardiac functions and tumor cell growth. Cell Tissue Res. 348, 281–94 (2012). human ICC-IF
Koeser, J., Troyanovsky, S. M., Grund, C. & Franke, W. W. De novo formation of desmosomes in cultured cells upon transfection of genes encoding specific desmosomal components. Exp. Cell Res. 285, 114–30 (2003). human ICC-IF
Chitaev, N. A. et al. The Binding of Plakoglobin to Desmosomal Cadherins: Patterns of Binding Sites and Topogenic Potential. J. Cell Biol. 113, 359–369 (1996). human ICC-IF
Achtstätter, T., Fouquet, B., Rungger‐BraUndele, E. & Frnake, W. W. Cytokeratin filaments and desmonsomes in the epithelioid cells of the perineurial and arachnoidal sheaths of some vertebrate species. Differentiation 40, 129–149 (1989). human IHC (frozen)
Rungger-Brandle, E., Achtstatter, T. & Franke, W. W. An epithelium-type cytoskeleton in a glial cell: Astrocytes of amphibian optic nerves contain cytokeratin filaments and are connected by desmosomes. J. Cell Biol. 109, 705–716 (1989). xenopus IHC (frozen)
Herrmann, H., Fouquet, B. & Franke, W. W. Expression of intermediate filament proteins during development of Xenopus laevis. I. cDNA clones encoding different forms of vimentin. Development 105, 279–98 (1989). xenopus IHC (frozen)
Franke, W. W. et al. Identification of the conserved, conformation-dependent cytokeratin epitope recognized by monoclonal antibody (lu-5). Virchows Arch. A Pathol. Anat. Histopathol. 411, 137–147 (1987). human, rat, bovine, chicken, xenopus IHC (frozen), ICC-IF, dot blot
Franke, W. W. & Moll, R. Cytoskeletal components of lymphoid organs. I. Synthesis of cytokeratins 8 and 18 and desmin in subpopulations of extrafollicular reticulum cells of human lymph nodes, tonsils, and spleen. Differentiation. 36, 145–63 (1987). human IHC (frozen)
Overbeck, J. et al. Immunohistochemical characterization of an anti-epithelial monoclonal antibody (mAB lu-5). Virchows Arch. A Pathol. Anat. Histopathol. 407, 1–12 (1985). human IHC (paraffin)

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