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mouse tongue (courtesy of J.Heß, University Hospital Heidelberg) Zoom

mouse tongue (courtesy of J.Heß, University Hospital Heidelberg)

anti-Keratin Pan mouse monoclonal, Lu-5, purified

Reacts with all types of epithelia and epithelium-derived neoplasms.
highly_published external_validation
Cat. No.: 610145
Quantity:  50 µg (lyoph.)

Delivery Time: usually 1-7 working days

$216.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone Lu-5
Immunogen cells of lung cancer cell line A549 and A2182
Purification affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Intended use Research use only
Tested species reactivity bovine, chicken, human, mouse, Pleurodelis, rat, snake, xenopus

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen assay dependent
Immunohistochemistry (IHC) - paraffin 1:10 - 1:20 (protease treatment and/or microwave treatment recommended)
Western Blot (WB) not applicable

Background

Details

Lu-5 represents an excellent marker for all types of epithelia. Ideal marker for immunolocalization studies on frozen and paraffin embedded tissue. Useful for distinguishing carcinomas from non-epithelial tumors.
Tumors specifically detected: all epithelium-derived neoplasms.

Polypeptide reacting: Most keratins present in human epithelia (including keratins – formerly also designated cytokeratins -K1, K5, K6, K14, K18, K19) and most acidic (K31 – KK38) and basic (K81 – K86) hair keratins from human hair follicle. The antibody specifically detects a conformational epitope common to most keratins, but not to other intermediate filament polypeptides.

Reactivity on cultured cell lines: MCF-7, PLC, A-431, HeLa; PtK2, A6 (XLKE).

Reference

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