Product description
Host | mouse |
---|---|
Antibody Type | monoclonal |
Isotype | IgG1 |
Clone | Lu-5 |
Immunogen | cells of lung cancer cell line A549 and A2182 |
Purification | affinity chromatography |
Conjugate | unconjugated |
Formulation | lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4) |
Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
No. of experiments | ≤ 400 (IHC, 50 µl/slide) |
Intended use | Research use only |
Tested species reactivity | bovine, chicken, human, mouse, Pleurodelis, rat, snake, xenopus |
Applications
Tested applications | Tested dilutions |
---|---|
Immunohistochemistry (IHC) - frozen | assay dependent |
Immunohistochemistry (IHC) - paraffin | 1:10 - 1:20 (protease treatment and/or microwave treatment recommended) |
Western Blot (WB) | not applicable |
Background
Details
Lu-5 represents an excellent marker for all types of epithelia. Ideal marker for immunolocalization studies on frozen and paraffin embedded tissue. Useful for distinguishing carcinomas from non-epithelial tumors.
Tumors specifically detected: all epithelium-derived neoplasms.
Polypeptide reacting: Most keratins present in human epithelia (including keratins – formerly also designated cytokeratins -K1, K5, K6, K14, K18, K19) and most acidic (K31 – KK38) and basic (K81 – K86) hair keratins from human hair follicle. The antibody specifically detects a conformational epitope common to most keratins, but not to other intermediate filament polypeptides.
Reactivity on cultured cell lines: MCF-7, PLC, A-431, HeLa; PtK2, A6 (XLKE).
Reference
Filter
rat
bovine
chicken
xenopus
ICC-IF
dot blot