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  • anti-Keratin Pan mouse monoclonal, Lu-5, purified

mouse tongue (courtesy of J.Heß, University Hospital Heidelberg) Zoom

mouse tongue (courtesy of J.Heß, University Hospital Heidelberg)

anti-Keratin Pan mouse monoclonal, Lu-5, purified

Reacts with all types of epithelia and epithelium-derived neoplasms.
highly_published external_validation
Cat. No.: 610145
Quantity:  50 µg (lyoph.)

Delivery Time: usually 1-7 working days

$216.00
Excl. 19% Tax, excl. Shipping Cost

Filter

Publication
Species
Application
Rickelt, S. Plakophilin-2: a cell-cell adhesion plaque molecule of selective and fundamental importance in cardiac functions and tumor cell growth. Cell Tissue Res. 348, 281-94 (2012).
human
ICC-IF
Koeser, J., Troyanovsky, S. M., Grund, C. & Franke, W. W. De novo formation of desmosomes in cultured cells upon transfection of genes encoding specific desmosomal components. Exp. Cell Res. 285, 114-30 (2003).
human
ICC-IF
Chitaev, N. A. et al. The Binding of Plakoglobin to Desmosomal Cadherins: Patterns of Binding Sites and Topogenic Potential. J. Cell Biol. 113, 359-369 (1996).
human
ICC-IF
Achtstätter, T., Fouquet, B., Rungger-BraUndele, E. & Frnake, W. W. Cytokeratin filaments and desmonsomes in the epithelioid cells of the perineurial and arachnoidal sheaths of some vertebrate species. Differentiation 40, 129-149 (1989).
human
IHC (frozen)
Rungger-Brandle, E., Achtstatter, T. & Franke, W. W. An epithelium-type cytoskeleton in a glial cell: Astrocytes of amphibian optic nerves contain cytokeratin filaments and are connected by desmosomes. J. Cell Biol. 109, 705-716 (1989).
xenopus
IHC (frozen)
Herrmann, H., Fouquet, B. & Franke, W. W. Expression of intermediate filament proteins during development of Xenopus laevis. I. cDNA clones encoding different forms of vimentin. Development 105, 279-98 (1989).
xenopus
IHC (frozen)
Franke, W. W. et al. Identification of the conserved, conformation-dependent cytokeratin epitope recognized by monoclonal antibody (lu-5). Virchows Arch. A Pathol. Anat. Histopathol. 411, 137-147 (1987).
human
rat
bovine
chicken
xenopus
IHC (frozen)
ICC-IF
dot blot
Franke, W. W. & Moll, R. Cytoskeletal components of lymphoid organs. I. Synthesis of cytokeratins 8 and 18 and desmin in subpopulations of extrafollicular reticulum cells of human lymph nodes, tonsils, and spleen. Differentiation. 36, 145-63 (1987).
human
IHC (frozen)
Overbeck, J. et al. Immunohistochemical characterization of an anti-epithelial monoclonal antibody (mAB lu-5). Virchows Arch. A Pathol. Anat. Histopathol. 407, 1-12 (1985).
human
IHC (paraffin)

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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone Lu-5
Immunogen cells of lung cancer cell line A549 and A2182
Purification affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Intended use Research use only
Tested species reactivity bovine, chicken, human, mouse, Pleurodelis, rat, snake, xenopus

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen assay dependent
Immunohistochemistry (IHC) - paraffin 1:10 - 1:20 (protease treatment and/or microwave treatment recommended)
Western Blot (WB) not applicable

Background

Details

Lu-5 represents an excellent marker for all types of epithelia. Ideal marker for immunolocalization studies on frozen and paraffin embedded tissue. Useful for distinguishing carcinomas from non-epithelial tumors.
Tumors specifically detected: all epithelium-derived neoplasms.

Polypeptide reacting: Most keratins present in human epithelia (including keratins – formerly also designated cytokeratins -K1, K5, K6, K14, K18, K19) and most acidic (K31 – KK38) and basic (K81 – K86) hair keratins from human hair follicle. The antibody specifically detects a conformational epitope common to most keratins, but not to other intermediate filament polypeptides.

Reactivity on cultured cell lines: MCF-7, PLC, A-431, HeLa; PtK2, A6 (XLKE).

Reference

Filter

Publication
Species
Application
Rickelt, S. Plakophilin-2: a cell-cell adhesion plaque molecule of selective and fundamental importance in cardiac functions and tumor cell growth. Cell Tissue Res. 348, 281-94 (2012).
human
ICC-IF
Koeser, J., Troyanovsky, S. M., Grund, C. & Franke, W. W. De novo formation of desmosomes in cultured cells upon transfection of genes encoding specific desmosomal components. Exp. Cell Res. 285, 114-30 (2003).
human
ICC-IF
Chitaev, N. A. et al. The Binding of Plakoglobin to Desmosomal Cadherins: Patterns of Binding Sites and Topogenic Potential. J. Cell Biol. 113, 359-369 (1996).
human
ICC-IF
Achtstätter, T., Fouquet, B., Rungger-BraUndele, E. & Frnake, W. W. Cytokeratin filaments and desmonsomes in the epithelioid cells of the perineurial and arachnoidal sheaths of some vertebrate species. Differentiation 40, 129-149 (1989).
human
IHC (frozen)
Rungger-Brandle, E., Achtstatter, T. & Franke, W. W. An epithelium-type cytoskeleton in a glial cell: Astrocytes of amphibian optic nerves contain cytokeratin filaments and are connected by desmosomes. J. Cell Biol. 109, 705-716 (1989).
xenopus
IHC (frozen)
Herrmann, H., Fouquet, B. & Franke, W. W. Expression of intermediate filament proteins during development of Xenopus laevis. I. cDNA clones encoding different forms of vimentin. Development 105, 279-98 (1989).
xenopus
IHC (frozen)
Franke, W. W. et al. Identification of the conserved, conformation-dependent cytokeratin epitope recognized by monoclonal antibody (lu-5). Virchows Arch. A Pathol. Anat. Histopathol. 411, 137-147 (1987).
human
rat
bovine
chicken
xenopus
IHC (frozen)
ICC-IF
dot blot
Franke, W. W. & Moll, R. Cytoskeletal components of lymphoid organs. I. Synthesis of cytokeratins 8 and 18 and desmin in subpopulations of extrafollicular reticulum cells of human lymph nodes, tonsils, and spleen. Differentiation. 36, 145-63 (1987).
human
IHC (frozen)
Overbeck, J. et al. Immunohistochemical characterization of an anti-epithelial monoclonal antibody (mAB lu-5). Virchows Arch. A Pathol. Anat. Histopathol. 407, 1-12 (1985).
human
IHC (paraffin)

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