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anti-Keratin Type I mouse monoclonal, AE1, purified

The antibody detects all epithelia and epithelium-derived neoplasms.
Cat. No.: 61804
Quantity:  200 µg (1 mg/mL)

Delivery Time: usually 1-7 working days

€217.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone AE1
Immunogen Human epidermal keratin (formerly also designated cytokeratin)
Purification affinity chromatography
Conjugate unconjugated
Formulation PBS pH 7.4 with 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, chicken, dog, human, monkey, mouse, rabbit, rat

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:200
Immunohistochemistry (IHC) - paraffin 1:200 (protease treatment and/or microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

AE 1 represents an excellent marker for distinguishing carcinoma from non-epithelial tumors. Tumors specifically detected: All epithelium-derived tumors.
Polypeptide reacting: Mr 40 000, Mr 48 000 and Mr 50 000 polypeptides of human epithelial proteins (keratins nos. K19, K16, K14) and most other acidic (type I) keratins.
Reactivity on cultured cell lines: MCF-7, A-431, RT 112

Reference

Reference

PublicationSpeciesApplication
Liang, F.-X. et al. Organization of uroplakin subunits: transmembrane topology, pair formation and plaque composition. Biochem. J. 355, 13–18 (2001). mouse, bovine IHC (paraffin)
Heid, H. W., Moll, I. & Franke, W. W. Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues. I. Human and bovine hair follicles. Differentiation. 37, 137–57 (1988). human, bovine IHC (frozen)
Heid, H. W., Moll, I. & Franke, W. W. Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues II. Concomitant and mutually exclusive synthesis of trichocytic and epithelial cytokeratins in diverse human and bovine tissues (hair follicle, nail bed and matrix, lingual papilla, thymic reticulum). Differentiation 37, 215–230 (1988). human IHC (frozen)
Chesa, P. G., Rettig, W. J. & Melamed, M. R. Expression of cytokeratins in normal and neoplastic colonic epithelial cells. Implications for cellular differentiation and carcinogenesis. Am. J. Surg. Pathol. 10, 829–835 (1986). human IHC (paraffin)
Spagnolo, D. V., Michie, S. A., Crabtree, G. S., Warnke, R. A. & Rouse, R. V. Monoclonal anti-keratin (AE1) reactivity in routinely processed tissue from 166 human neoplasms. Am. J. Clin. Pathol. 84, 697–704 (1985). human IHC (paraffin)
Battifora, H., Sheibani, K., Tubbs, R., Kopinski, M. & Sun, T.-T. Antikeratin antibodies in tumor diagnosis. Cancer 54, 843–848 (1984). human IHC (frozen, paraffin)
Nelson, W. G., Battifora, H. & Sun, T. T. Specific Keratins as Molecular Markers for Neoplasms with a Stratified Epithelial Origin. Cancer Res. 44, 1600–1603 (1984). human WB
Eichner, R., Bonitz, P. & Sun, T. T. Classification of epidermal keratins according to their immunoreactivity, isoelectric point, and mode of expression. J. Cell Biol. 98, 1388–1396 (1984). human WB
Shi, S. R. et al. Immunohistochemical study of nasopharyngeal carcinoma using monoclonal keratin antibodies. Am. J. Pathol. 117, 53–63 (1984). human IHC (paraffin)
Weiss, R. A., Eichner, R. & Sun, T. T. Monoclonal antibody analysis of keratin expression in epidermal diseases: A 48- and 56-kdalton keratin as molecular markers for hyperproliferative keratinocytes. J. Cell Biol. 98, 1397–1406 (1984). human WB
Weiss, R. a et al. The use of monoclonal antibody to keratin in human epidermal disease: alterations in immunohistochemical staining pattern. J. Invest. Dermatol. 81, 224–30 (1983). human IHC (frozen), ICC-IF
Sun, T. T. et al. Keratin classes: molecular markers for different types of epithelial differentiation. J Invest Dermatol 81, 109s–15s (1983). human IHC (frozen), ICC-IF
Nelson, W. G. & Sun, T. T. The 50- and 58-kdalton keratin classes as molecular markers for stratified squamous epithelia: Cell culture studies. J. Cell Biol. 97, 244–251 (1983). human WB, ICC-IF
Tseng, S. C. G. et al. Correlation of specific keratins with different types of epithelial differentiation: Monoclonal antibody studies. Cell 30, 361–372 (1982). human, monkey, rabbit WB, IHC (frozen), ICC-IF
Woodcock-Mitchell, J., Eichner, R., Nelson, W. G. & Sun, T. T. Immunolocalization of Keratin Polypeptides in Human-Epidermis Using Monoclonal-Antibodies. J. Cell Biol. 95, 580–588 (1982). human WB, IHC (frozen), ICC-IF

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