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  • anti-Keratin Type I mouse monoclonal, AE1, purified

anti-Keratin Type I mouse monoclonal, AE1, purified Zoom

anti-Keratin Type I mouse monoclonal, AE1, purified

The antibody detects all epithelia and epithelium-derived neoplasms.
Cat. No.: 61804
Quantity:  200 µg (1 mg/mL)
No. of experiments:  ≤ 800 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

$271.00
Excl. VAT, excl. Shipping Cost

Filter

Publication
Species
Application
Orós, J. et al. Immunohistochemical staining patterns of alpha-keratins in normal tissues from two reptile species: implications for characterization of squamous cell carcinomas. BMC.Vet.Res. 14, 219 (2018).
reptile
IHC (paraffin)
Liang, F.-X. et al. Organization of uroplakin subunits: transmembrane topology, pair formation and plaque composition. Biochem. J. 355, 13–18 (2001).
mouse
IHC (paraffin)
Heid, H. W., Moll, I. & Franke, W. W. Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues. I. Human and bovine hair follicles. Differentiation. 37, 137–57 (1988).
human
IHC (frozen)
Heid, H. W., Moll, I. & Franke, W. W. Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues II. Concomitant and mutually exclusive synthesis of trichocytic and epithelial cytokeratins in diverse human and bovine tissues (h
human
IHC (frozen)
Chesa, P. G., Rettig, W. J. & Melamed, M. R. Expression of cytokeratins in normal and neoplastic colonic epithelial cells. Implications for cellular differentiation and carcinogenesis. Am. J. Surg. Pathol. 10, 829–835 (1986).
human
IHC (paraffin)
Spagnolo, D. V., Michie, S. A., Crabtree, G. S., Warnke, R. A. & Rouse, R. V. Monoclonal anti-keratin (AE1) reactivity in routinely processed tissue from 166 human neoplasms. Am. J. Clin. Pathol. 84, 697–704 (1985).
human
IHC (paraffin)
Battifora, H., Sheibani, K., Tubbs, R., Kopinski, M. & Sun, T.-T. Antikeratin antibodies in tumor diagnosis. Cancer 54, 843–848 (1984).
human
IHC (frozen)
IHC (paraffin)
Nelson, W. G., Battifora, H. & Sun, T. T. Specific Keratins as Molecular Markers for Neoplasms with a Stratified Epithelial Origin. Cancer Res. 44, 1600–1603 (1984).
human
WB
Eichner, R., Bonitz, P. & Sun, T. T. Classification of epidermal keratins according to their immunoreactivity, isoelectric point, and mode of expression. J. Cell Biol. 98, 1388–1396 (1984).
human
WB
Shi, S. R. et al. Immunohistochemical study of nasopharyngeal carcinoma using monoclonal keratin antibodies. Am. J. Pathol. 117, 53–63 (1984).
human
IHC (paraffin)
Weiss, R. A., Eichner, R. & Sun, T. T. Monoclonal antibody analysis of keratin expression in epidermal diseases: A 48- and 56-kdalton keratin as molecular markers for hyperproliferative keratinocytes. J. Cell Biol. 98, 1397–1406 (1984).
human
WB
Weiss, R. a et al. The use of monoclonal antibody to keratin in human epidermal disease: alterations in immunohistochemical staining pattern. J. Invest. Dermatol. 81, 224–30 (1983).
human
IHC (frozen)
ICC-IF
Sun, T. T. et al. Keratin classes: molecular markers for different types of epithelial differentiation. J Invest Dermatol 81, 109s–15s (1983).
human
IHC (frozen)
ICC-IF
Nelson, W. G. & Sun, T. T. The 50- and 58-kdalton keratin classes as molecular markers for stratified squamous epithelia: Cell culture studies. J. Cell Biol. 97, 244–251 (1983).
human
WB
ICC-IF
Tseng, S. C. G. et al. Correlation of specific keratins with different types of epithelial differentiation: Monoclonal antibody studies. Cell 30, 361–372 (1982).
human
monkey
rabbit
WB
IHC (frozen)
ICC-IF
Woodcock-Mitchell, J., Eichner, R., Nelson, W. G. & Sun, T. T. Immunolocalization of Keratin Polypeptides in Human-Epidermis Using Monoclonal-Antibodies. J. Cell Biol. 95, 580–588 (1982).
human
WB
IHC (frozen)
ICC-IF

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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone AE1
Immunogen Human epidermal keratin (formerly also designated cytokeratin)
Purification affinity chromatography
Conjugate unconjugated
Formulation PBS pH 7.4 with 0.09% sodium azide
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 800 (IHC, 50 µl/slide)
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity bovine, chicken, dog, human, monkey, mouse, rabbit, rat

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:200
Immunohistochemistry (IHC) - paraffin 1:200 (protease treatment and/or microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

AE 1 represents an excellent marker for distinguishing carcinoma from non-epithelial tumors. Tumors specifically detected: All epithelium-derived tumors.
Polypeptide reacting: Mr 40 000, Mr 48 000 and Mr 50 000 polypeptides of human epithelial proteins (keratins nos. K19, K16, K14) and most other acidic (type I) keratins.
Reactivity on cultured cell lines: MCF-7, A-431, RT 112

Reference

Filter

Publication
Species
Application
Orós, J. et al. Immunohistochemical staining patterns of alpha-keratins in normal tissues from two reptile species: implications for characterization of squamous cell carcinomas. BMC.Vet.Res. 14, 219 (2018).
reptile
IHC (paraffin)
Liang, F.-X. et al. Organization of uroplakin subunits: transmembrane topology, pair formation and plaque composition. Biochem. J. 355, 13–18 (2001).
mouse
IHC (paraffin)
Heid, H. W., Moll, I. & Franke, W. W. Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues. I. Human and bovine hair follicles. Differentiation. 37, 137–57 (1988).
human
IHC (frozen)
Heid, H. W., Moll, I. & Franke, W. W. Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues II. Concomitant and mutually exclusive synthesis of trichocytic and epithelial cytokeratins in diverse human and bovine tissues (h
human
IHC (frozen)
Chesa, P. G., Rettig, W. J. & Melamed, M. R. Expression of cytokeratins in normal and neoplastic colonic epithelial cells. Implications for cellular differentiation and carcinogenesis. Am. J. Surg. Pathol. 10, 829–835 (1986).
human
IHC (paraffin)
Spagnolo, D. V., Michie, S. A., Crabtree, G. S., Warnke, R. A. & Rouse, R. V. Monoclonal anti-keratin (AE1) reactivity in routinely processed tissue from 166 human neoplasms. Am. J. Clin. Pathol. 84, 697–704 (1985).
human
IHC (paraffin)
Battifora, H., Sheibani, K., Tubbs, R., Kopinski, M. & Sun, T.-T. Antikeratin antibodies in tumor diagnosis. Cancer 54, 843–848 (1984).
human
IHC (frozen)
IHC (paraffin)
Nelson, W. G., Battifora, H. & Sun, T. T. Specific Keratins as Molecular Markers for Neoplasms with a Stratified Epithelial Origin. Cancer Res. 44, 1600–1603 (1984).
human
WB
Eichner, R., Bonitz, P. & Sun, T. T. Classification of epidermal keratins according to their immunoreactivity, isoelectric point, and mode of expression. J. Cell Biol. 98, 1388–1396 (1984).
human
WB
Shi, S. R. et al. Immunohistochemical study of nasopharyngeal carcinoma using monoclonal keratin antibodies. Am. J. Pathol. 117, 53–63 (1984).
human
IHC (paraffin)
Weiss, R. A., Eichner, R. & Sun, T. T. Monoclonal antibody analysis of keratin expression in epidermal diseases: A 48- and 56-kdalton keratin as molecular markers for hyperproliferative keratinocytes. J. Cell Biol. 98, 1397–1406 (1984).
human
WB
Weiss, R. a et al. The use of monoclonal antibody to keratin in human epidermal disease: alterations in immunohistochemical staining pattern. J. Invest. Dermatol. 81, 224–30 (1983).
human
IHC (frozen)
ICC-IF
Sun, T. T. et al. Keratin classes: molecular markers for different types of epithelial differentiation. J Invest Dermatol 81, 109s–15s (1983).
human
IHC (frozen)
ICC-IF
Nelson, W. G. & Sun, T. T. The 50- and 58-kdalton keratin classes as molecular markers for stratified squamous epithelia: Cell culture studies. J. Cell Biol. 97, 244–251 (1983).
human
WB
ICC-IF
Tseng, S. C. G. et al. Correlation of specific keratins with different types of epithelial differentiation: Monoclonal antibody studies. Cell 30, 361–372 (1982).
human
monkey
rabbit
WB
IHC (frozen)
ICC-IF
Woodcock-Mitchell, J., Eichner, R., Nelson, W. G. & Sun, T. T. Immunolocalization of Keratin Polypeptides in Human-Epidermis Using Monoclonal-Antibodies. J. Cell Biol. 95, 580–588 (1982).
human
WB
IHC (frozen)
ICC-IF

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