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anti-Keratin Type I mouse monoclonal, AE1, purified

The antibody detects all epithelia and epithelium-derived neoplasms.
Cat. No.: 61804
Quantity:  200 µg (1 mg/mL)
No. of experiments:  ≤ 800 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone AE1
Immunogen Human epidermal keratin (formerly also designated cytokeratin)
Purification affinity chromatography
Conjugate unconjugated
Formulation PBS pH 7.4 with 0.09% sodium azide
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 800 (IHC, 50 µl/slide)
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity bovine, chicken, dog, human, monkey, mouse, rabbit, rat


Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:200
Immunohistochemistry (IHC) - paraffin 1:200 (protease treatment and/or microwave treatment recommended)
Western Blot (WB) assay dependent



AE 1 represents an excellent marker for distinguishing carcinoma from non-epithelial tumors. Tumors specifically detected: All epithelium-derived tumors.
Polypeptide reacting: Mr 40 000, Mr 48 000 and Mr 50 000 polypeptides of human epithelial proteins (keratins nos. K19, K16, K14) and most other acidic (type I) keratins.
Reactivity on cultured cell lines: MCF-7, A-431, RT 112


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