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anti-Keratin Type II mouse monoclonal, AE3, purified

The antibody detects all epithelia and epithelium-derived neoplasms.

Cat. No.: 61806
Quantity:  200 µg

Delivery Time: usually 1-7 working days

€217.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone AE3
Immunogen Human epidermal keratin
Purification affinity chromatography
Conjugate unconjugated
Formulation PBS pH 7.4 with 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, chicken, human, monkey, mouse, rabbit, rat
Intended use Research use only

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:100
Immunohistochemistry (IHC) - paraffin 1:100 (protease treatment and/or microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

AE 3 represents an excellent marker for distinguishing carcinoma from non-epithelial tumors. Tumors specifically detected: All epithelia and their neoplasms.
Polypeptide reacting: Mr 53 000 to Mr 67 000 basic type II keratin polypeptides of human epithelial proteins (keratins – formerly also designated cytokeratins - nos.
K1 – K8). Epitope has been mapped to aa 266-299 on alpha-helical rod domain (see Waseem et al., 2004).
Reactivity on cultured cell lines: MCF-7, A-431, RT 112


Waseem A, Karsten U, Leigh IM, Purkis P, Waseem NH, Lane BE: Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability. Biochemistry 43, 1283-1295 (2004)

Reference

Reference

PublicationSpeciesApplication
Liang, F.-X. et al. Organization of uroplakin subunits: transmembrane topology, pair formation and plaque composition. Biochem. J. 355, 13–18 (2001). mouse, bovine IHC (paraffin)
Nelson, W. G., Battifora, H. & Sun, T. T. Specific Keratins as Molecular Markers for Neoplasms with a Stratified Epithelial Origin. Cancer Res. 44, 1600–1603 (1984). human WB
Eichner, R., Bonitz, P. & Sun, T. T. Classification of epidermal keratins according to their immunoreactivity, isoelectric point, and mode of expression. J. Cell Biol. 98, 1388–1396 (1984). human WB
Shi, S. R. et al. Immunohistochemical study of nasopharyngeal carcinoma using monoclonal keratin antibodies. Am. J. Pathol. 117, 53–63 (1984). human IHC (paraffin)
Weiss, R. A., Eichner, R. & Sun, T. T. Monoclonal antibody analysis of keratin expression in epidermal diseases: A 48- and 56-kdalton keratin as molecular markers for hyperproliferative keratinocytes. J. Cell Biol. 98, 1397–1406 (1984). human WB
Sun, T. T. et al. Keratin classes: molecular markers for different types of epithelial differentiation. J Invest Dermatol 81, 109s–15s (1983). human IHC (frozen), ICC-IF
Nelson, W. G. & Sun, T. T. The 50- and 58-kdalton keratin classes as molecular markers for stratified squamous epithelia: Cell culture studies. J. Cell Biol. 97, 244–251 (1983). human WB, ICC-IF
Tseng, S. C. G. et al. Correlation of specific keratins with different types of epithelial differentiation: Monoclonal antibody studies. Cell 30, 361–372 (1982). human, monkey WB, ICC-IF
Woodcock-Mitchell, J., Eichner, R., Nelson, W. G. & Sun, T. T. Immunolocalization of Keratin Polypeptides in Human-Epidermis Using Monoclonal-Antibodies. J. Cell Biol. 95, 580–588 (1982). human WB, ICC-IF

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