Call +49 6221 8278-0

PROGEN - passion for research


anti-Keratin Type II mouse monoclonal, AE3, purified

The antibody detects all epithelia and epithelium-derived neoplasms.

Cat. No.: 61806
Quantity:  200 µg
No. of experiments:  ≤ 400 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

Excl. VAT, excl. Shipping Cost

Call us now for more information on our products: +49 6221 8278-0

Any more questions?


Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone AE3
Immunogen Human epidermal keratin
Purification affinity chromatography
Conjugate unconjugated
Formulation PBS pH 7.4 with 0.09% sodium azide
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 400 (IHC, 50 µl/slide)
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity bovine, chicken, human, monkey, mouse, rabbit, rat


Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:100
Immunohistochemistry (IHC) - paraffin 1:100 (protease treatment and/or microwave treatment recommended)
Western Blot (WB) assay dependent



AE 3 represents an excellent marker for distinguishing carcinoma from non-epithelial tumors. Tumors specifically detected: All epithelia and their neoplasms.
Polypeptide reacting: Mr 53 000 to Mr 67 000 basic type II keratin polypeptides of human epithelial proteins (keratins – formerly also designated cytokeratins - nos.
K1 – K8). Epitope has been mapped to aa 266-299 on alpha-helical rod domain (see Waseem et al., 2004).
Reactivity on cultured cell lines: MCF-7, A-431, RT 112

Waseem A, Karsten U, Leigh IM, Purkis P, Waseem NH, Lane BE: Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability. Biochemistry 43, 1283-1295 (2004)


Material safety data sheet

Please complete the form below to request a material safety data sheet

Personal Info

* Required Fields

Product Request

Personal Info

* Required Fields