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Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG1 |
| Clone | AE3 |
| Immunogen | Human epidermal keratin |
| Purification | affinity chromatography |
| Conjugate | unconjugated |
| Formulation | PBS pH 7.4 with 0.09% sodium azide |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 400 (IHC, 50 µl/slide) |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | bovine, chicken, human, monkey, mouse, rabbit, rat |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:100 |
| Immunohistochemistry (IHC) - paraffin | 1:100 (protease treatment and/or microwave treatment recommended) |
| Western Blot (WB) | assay dependent |
Background
Details
AE 3 represents an excellent marker for distinguishing carcinoma from non-epithelial tumors. Tumors specifically detected: All epithelia and their neoplasms.
Polypeptide reacting: Mr 53 000 to Mr 67 000 basic type II keratin polypeptides of human epithelial proteins (keratins – formerly also designated cytokeratins - nos.
K1 – K8). Epitope has been mapped to aa 266-299 on alpha-helical rod domain (see Waseem et al., 2004).
Reactivity on cultured cell lines: MCF-7, A-431, RT 112
Waseem A, Karsten U, Leigh IM, Purkis P, Waseem NH, Lane BE: Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability. Biochemistry 43, 1283-1295 (2004)
Reference
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Species
Application
mouse
IHC (paraffin)
human
IHC (paraffin)
human
IHC (frozen)
ICC-IF
ICC-IF
human
monkey
monkey
WB
ICC-IF
ICC-IF
