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anti-Keratin Type II mouse monoclonal, AE3, purified

The antibody detects all epithelia and epithelium-derived neoplasms.

Cat. No.: 61806
Quantity:  200 µg

Delivery Time: usually 1-7 working days

$271.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone AE3
Immunogen Human epidermal keratin
Purification affinity chromatography
Conjugate unconjugated
Formulation PBS pH 7.4 with 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity bovine, chicken, human, monkey, mouse, rabbit, rat

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:100
Immunohistochemistry (IHC) - paraffin 1:100 (protease treatment and/or microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

AE 3 represents an excellent marker for distinguishing carcinoma from non-epithelial tumors. Tumors specifically detected: All epithelia and their neoplasms.
Polypeptide reacting: Mr 53 000 to Mr 67 000 basic type II keratin polypeptides of human epithelial proteins (keratins – formerly also designated cytokeratins - nos.
K1 – K8). Epitope has been mapped to aa 266-299 on alpha-helical rod domain (see Waseem et al., 2004).
Reactivity on cultured cell lines: MCF-7, A-431, RT 112


Waseem A, Karsten U, Leigh IM, Purkis P, Waseem NH, Lane BE: Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability. Biochemistry 43, 1283-1295 (2004)

Reference

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