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IHC of human HNSCC tissue (courtesy of J.Heß, University Hospital Heidelberg) Zoom

IHC of human HNSCC tissue (courtesy of J.Heß, University Hospital Heidelberg)

anti-Keratin Type II mouse monoclonal, Ks pan1-8, lyophilized, purified

The antibody detects all epithelia and epithelium-derived neoplasms.
highly_published external_validation
Cat. No.: 61006
Quantity:  50 µg
No. of experiments:  ≤ 2000 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2a
Clone Ks pan1-8
Immunogen cytoskeletal proteins from cultured human MCF-7 cells
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 2000 (IHC, 50 µl/slide)
Intended use Research use only
Tested species reactivity amphibia, bovine, human, mouse, rat


Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:10 - 1:100
Immunohistochemistry (IHC) - paraffin 1:10 - 1:100 (microwave treatment recommended)
Western Blot (WB) assay dependent



Ks pan1-8 represents an excellent marker for distinguishing carcinomas from non-epithelial tumors.
Polypeptides reacting: Mr 52 500 - Mr 68 000 keratins (type II keratins K1 - K8; formerly also designated cytokeratins 1 - 8) of human epithelial cells.
Tumors specifically detected: all epithelium-derived neoplasms.

Reactivity on cultured cell lines MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650, SSC-12.


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