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Product description
Host | mouse |
---|---|
Antibody Type | monoclonal |
Isotype | IgG2a |
Clone | Ks pan1-8 |
Immunogen | cytoskeletal proteins from cultured human MCF-7 cells |
Purification | protein A affinity chromatography |
Conjugate | unconjugated |
Formulation | lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4) |
Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
No. of experiments | ≤ 2000 (IHC, 50 µl/slide) |
Intended use | Research use only |
Tested species reactivity | amphibia, bovine, human, mouse, rat |
Applications
Tested applications | Tested dilutions |
---|---|
Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
Immunohistochemistry (IHC) - frozen | 1:10 - 1:100 |
Immunohistochemistry (IHC) - paraffin | 1:10 - 1:100 (microwave treatment recommended) |
Western Blot (WB) | assay dependent |
Background
Details
Ks pan1-8 represents an excellent marker for distinguishing carcinomas from non-epithelial tumors.
Polypeptides reacting: Mr 52 500 - Mr 68 000 keratins (type II keratins K1 - K8; formerly also designated cytokeratins 1 - 8) of human epithelial cells.
Tumors specifically detected: all epithelium-derived neoplasms.
Reactivity on cultured cell lines MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650, SSC-12.
Reference
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Species
Application
zebrafish
whole mount
zebrafish
whole mount
zebrafish
whole mount
human
xenopus
xenopus
WB
IHC (frozen)
IHC (frozen)