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anti-Lamin B mouse monoclonal, X223, lyophilized, purified

The monoclonal antibody decorates the karyoskeleton, i.e. the intermediate filament equivalent of the nucleus. Specific reaction with lamin B.
Cat. No.: 61047C
Quantity:  50 µg
No. of experiments:  ≤ 200 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

$251.00
Excl. VAT, excl. Shipping Cost

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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone X223
Immunogen Nuclear pore complex-lamina fraction of Xenopus laevis (XLKE-A6 cells)
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 200 (IHC, 50 µl/slide)
Intended use Research use only
Tested species reactivity bovine, human, mouse, rat, rat kangoroo, trout

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:10
Immunohistochemistry (IHC) - frozen 1:10
Western Blot (WB) assay dependent

Background

Details

The monoclonal antibody decorates the karyoskeleton, i.e. the intermediate filament equivalent of the nucleus.
The epitope was localized to coil 1B (Schumacher et al. 2006).
Polypeptides reacting: Lamin isotypes of Mr 60 - 75 kD

Antigen recognized in species:

Xenopus laevis       LII, LIII
bovine LB1, B2
mouse LB2
rat +
human LB1, B2
trout LII, LIII
rat kangaroo LII, LIII

Schumacher, J., Reichenzeller, M., Kempf, T., Schnölzer, M. & Herrmann, H. Identification of a novel, highly variable amino-terminal amino acid sequence element in the nuclear intermediate filament protein lamin B2 from higher vertebrates. FEBS Lett. 580, 6211–6216 (2006).

Reference

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