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anti-Lamin mouse monoclonal, X67, lyophilized, purified

The monoclonal antibody decorates the karyoskeleton, i.e. the intermediate filament equivalent of the nucleus. Specific reaction with lamin A and C.
Cat. No.: 61047A
Quantity:  50µg
No. of experiments:  ≤ 200 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

$251.00
Excl. VAT, excl. Shipping Cost

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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone X67
Immunogen Nuclear pore complex-lamina fraction of Xenopus laevis (XLKE-A6 cells)
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 200 (IHC, 50 µl/slide)
Intended use Research use only
Tested species reactivity bovine, human, mouse, rat, rat kangoroo, trout (negativ with rat and rat kangaroo)

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:10
Immunohistochemistry (IHC) - frozen 1:10
Western Blot (WB) assay dependent

Background

Details

The monoclonal antibody cocktail decorates the karyoskeleton, i.e. the intermediate filament equivalent of the nucleus.
Polypeptides reacting: Lamin isotypes of Mr 60 - 75 kD

Antigen recognized in species:

Xenopus laevis       LA, LI, LII
bovine LA, C
mouse n.d.
rat -
human LA, C
trout LA, LI, LII
rat kangaroo -

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