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Human, Kidney Zoom

Human, Kidney

anti-Laminin rabbit polyclonal, serum

Reacts with 200 kD glycoprotein Laminin of basement membranes; for studies of cell adhesion and cell attachment in vivo and in vitro.
Cat. No.: 10765
Quantity:  250 µL

Delivery Time: usually 1-7 working days

€234.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

Product description

Host rabbit
Antibody Type polyclonal
Immunogen Laminin isolated from Engelbrecht-Holm-Swarm (EHS)-mouse sarcoma
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity bovine, human, mouse, pig, rabbit, rat (most mammals)

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:50 – 1:100
Immunohistochemistry (IHC) - paraffin 1:50 – 1:100 (proteolytic treatment required; enzyme: pepsin)
Western Blot (WB) assay dependent

Background

Details

Laminin is a glycoprotein (Mr 850 - 1.000 kD, consisting of 3 glycosylated polypeptide chains with molecular weights of 440 and 225 (2x) kD) produced by various human epithelial and mesenchymal cells, and forms an extracellular matrix of thin filaments. In normal tissues, laminin is invariably present in all basal laminas surrounding muscle, nerve, fat and decidua cells and separates epithelial and endothelial cells from abutting connective tissues. Laminin has also been identified within the cytoplasm of breast epithelia, stromal cells of the endometrium, and within endothelial, bile duct epithelial and mesenchymal cells of the liver. Laminin has been found to be involved in cellular activities such as adhesion, spreading, differentiation, polarization, proliferation, locomotion, tissue invasion and chemotactic responses.
No cross reaction was obtained with human type I, III, IV and V collagen in immunoblotting, whereas the antibody reacted with a distinct band of appr. 200-220 kD from a 8M Urea extract from amnion basement membrane.

Positive control: skin, kidney.

Reference

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