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Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG2b kappa |
| Clone | IgG-C7 |
| Immunogen | purified bovine adrenal cortex LDL receptor |
| Purification | protein A affinity chromatography |
| Conjugate | unconjugated |
| Formulation | lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.5% BSA in PBS buffer, pH 7.4) |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| UniprotID | P01131 |
| No. of experiments | ≤ 2000 (IHC, 50 µl/slide) |
| Intended use | Research use only |
| Tested species reactivity | bovine, human (negative with rat, mouse, hamster (chinese hamster ovary cells), dog, rabbit) |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Flow Cytometry (FACS) | assay dependent |
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | 1:10 – 1:100 |
| Immunohistochemistry (IHC) - paraffin | 1:100 |
Background
Details
Suitable for analysis of LDL receptor function in patients with familial hypercholesterolemia. The antibody recognizes an epitope in the region of repeat #1 of the ligand binding region. Addition of 15 nM antibody results in inhibition of half-maximal LDL-binding (Beisiegel et al. 1981). In human normal fibroblasts the antibody detects the 160 kD polypeptide (pI 4.3) and also in bovine adrenal gland (160 kD; pI 4.6) of LDL receptors (Beisiegel et al. 1982).
Beisiegelt, U., Schneider, W. J., Brown, M. S. & Goldstein, J. L. Immunoblot Analysis of Low Density Lipoprotein Receptors in Fibroblasts from Subjects with Familial Hypercholesterolemia. J. Biol. Chem. 257, 13150–13156 (1982).
Beisiegel, U., Schneider, W. J., Goldstein, J. L., Anderson, R. G. & Brown, M. S. Monoclonal antibodies to the low density lipoprotein receptor as probes for study of receptor-mediated endocytosis and the genetics of familial hypercholesterolemia. J. Biol. Chem. 256, 11923–31 (1981).
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