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anti-LDL Receptor mouse monoclonal, IgG-C7, lyophilized, purified

The antibody is useful for the analysis of LDL receptor function in patients with familial hypercholesterolemia in Immunoblotting.
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Cat. No.: 61087
Quantity:  50 µg

Delivery Time: usually 1-7 working days

$257.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2b kappa
Clone IgG-C7
Immunogen purified bovine adrenal cortex LDL receptor
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
UniprotID P01131
Intended use Research use only
Tested species reactivity bovine, human (negative with rat, mouse, hamster (chinese hamster ovary cells), dog, rabbit)

Applications

Tested applications Tested dilutions
Flow Cytometry (FACS) assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:10 – 1:100
Immunohistochemistry (IHC) - paraffin 1:100

Background

Details

Suitable for analysis of LDL receptor function in patients with familial hypercholesterolemia. The antibody recognizes an epitope in the region of repeat #1 of the ligand binding region. Addition of 15 nM antibody results in inhibition of half-maximal LDL-binding (Beisiegel et al. 1981). In human normal fibroblasts the antibody detects the 160 kD polypeptide (pI 4.3) and also in bovine adrenal gland (160 kD; pI 4.6) of LDL receptors (Beisiegel et al. 1982).

Beisiegelt, U., Schneider, W. J., Brown, M. S. & Goldstein, J. L. Immunoblot Analysis of Low Density Lipoprotein Receptors in Fibroblasts from Subjects with Familial Hypercholesterolemia. J. Biol. Chem. 257, 13150–13156 (1982).
Beisiegel, U., Schneider, W. J., Goldstein, J. L., Anderson, R. G. & Brown, M. S. Monoclonal antibodies to the low density lipoprotein receptor as probes for study of receptor-mediated endocytosis and the genetics of familial hypercholesterolemia. J. Biol. Chem. 256, 11923–31 (1981).

Reference

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