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human liver (courtesy of J.Heß, University Hospital Heidelberg) Zoom

human liver (courtesy of J.Heß, University Hospital Heidelberg)

anti-LDL Receptor rabbit polyclonal, serum

The antibody is raised against a specific synthetic peptide of the LDLR extracellular domain (epitope between residues Nos. 184-195, the linker region between repeats 4 and 5).
application_confirmed external_validation
Cat. No.: 61099
Quantity:  250 µL (0.1 mg/mL)

Delivery Time: usually 1-7 working days

$305.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

Alternative Products

Cat. No. Product Name Format Quantity Price
61599

anti-LDL Receptor rabbit polyclonal

Biotin Conjugate 250 µL
$391.00
Excl. 19% Tax, excl. Shipping Cost

Product description

Host rabbit
Antibody Type polyclonal
Isotype IgG
Immunogen specific synthetic peptide (sequence not conserved in VLDL receptor and LRP) of the human LDL receptor extracellular domain (epitope between residues nos. 184-195, the linker region between repeats 4 and 5)
Purification affinity chromatography
Conjugate unconjugated
Formulation PBS with 0.5% BSA
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity bovine, human, mouse, rat

Applications

Tested applications Tested dilutions
Flow Cytometry (FACS) 1:10
Immunohistochemistry (IHC) - frozen 1:20 - 1:50
Immunohistochemistry (IHC) - paraffin 1:20 - 1:50 (microwave treatment recommended)
Receptor Binding Assays assay-dependent
Western Blot (WB) 1:100 – 1:200

Background

Details

The LDL receptor (160 kD mature or glycosylated receptor; 120 kD precursor or unglycosylated receptor) plays a key role in cellular cholesterol homeostasis.
The antibody to LDLR reacts specifically with human LDLR and is suitable for use in flow cytometry and immunoblotting. The antibody does not inhibit binding of LDL.
In immunoblotting, the antibody recognizes the 160 kD band of LDLR and a 120 kD band of the LDLR precursor from fibroblasts, hepatocytes, and monocytic cells cultured in the presence of lipoprotein-deficient serum.

Reference

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